TY - JOUR

T1 - Effect of the relief on the measurement of bond rupture force with the help of AFM

T2 - The dynamics of interaction and optimization of the procedure

AU - Kurus, N. N.

AU - Dultsev, F. N.

AU - Shevelev, G.

AU - Lomzov, A. A.

AU - Pyshnyi, D. V.

PY - 2018/7/28

Y1 - 2018/7/28

N2 - Measurement of the forces of unwinding of DNA double helix was conducted. Silicon wafers were chosen as substrates. It is demonstrated that the use of gold for surface modification causes the bimodal probability distribution of rupture forces because of the surface roughness. Surface modification with the help of Langmuir-Blodgett procedure is proposed as an alternative. The use of this method gives unimodal distribution of rupture forces. The forces of double helix unwinding are reported for oligonucleotides containing 20 base pairs depending on salt concentration. Furthermore, forces for the shear mode and unzipping mode are measured.

AB - Measurement of the forces of unwinding of DNA double helix was conducted. Silicon wafers were chosen as substrates. It is demonstrated that the use of gold for surface modification causes the bimodal probability distribution of rupture forces because of the surface roughness. Surface modification with the help of Langmuir-Blodgett procedure is proposed as an alternative. The use of this method gives unimodal distribution of rupture forces. The forces of double helix unwinding are reported for oligonucleotides containing 20 base pairs depending on salt concentration. Furthermore, forces for the shear mode and unzipping mode are measured.

UR - http://www.scopus.com/inward/record.url?scp=85050274584&partnerID=8YFLogxK

U2 - 10.1039/c8ay00821c

DO - 10.1039/c8ay00821c

M3 - Article

AN - SCOPUS:85050274584

VL - 10

SP - 3498

EP - 3505

JO - Analytical Methods

JF - Analytical Methods

SN - 1759-9660

IS - 28

ER -