Research output: Contribution to journal › Article › peer-review
Effect of the relief on the measurement of bond rupture force with the help of AFM : The dynamics of interaction and optimization of the procedure. / Kurus, N. N.; Dultsev, F. N.; Shevelev, G. et al.
In: Analytical Methods, Vol. 10, No. 28, 28.07.2018, p. 3498-3505.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Effect of the relief on the measurement of bond rupture force with the help of AFM
T2 - The dynamics of interaction and optimization of the procedure
AU - Kurus, N. N.
AU - Dultsev, F. N.
AU - Shevelev, G.
AU - Lomzov, A. A.
AU - Pyshnyi, D. V.
PY - 2018/7/28
Y1 - 2018/7/28
N2 - Measurement of the forces of unwinding of DNA double helix was conducted. Silicon wafers were chosen as substrates. It is demonstrated that the use of gold for surface modification causes the bimodal probability distribution of rupture forces because of the surface roughness. Surface modification with the help of Langmuir-Blodgett procedure is proposed as an alternative. The use of this method gives unimodal distribution of rupture forces. The forces of double helix unwinding are reported for oligonucleotides containing 20 base pairs depending on salt concentration. Furthermore, forces for the shear mode and unzipping mode are measured.
AB - Measurement of the forces of unwinding of DNA double helix was conducted. Silicon wafers were chosen as substrates. It is demonstrated that the use of gold for surface modification causes the bimodal probability distribution of rupture forces because of the surface roughness. Surface modification with the help of Langmuir-Blodgett procedure is proposed as an alternative. The use of this method gives unimodal distribution of rupture forces. The forces of double helix unwinding are reported for oligonucleotides containing 20 base pairs depending on salt concentration. Furthermore, forces for the shear mode and unzipping mode are measured.
UR - http://www.scopus.com/inward/record.url?scp=85050274584&partnerID=8YFLogxK
U2 - 10.1039/c8ay00821c
DO - 10.1039/c8ay00821c
M3 - Article
AN - SCOPUS:85050274584
VL - 10
SP - 3498
EP - 3505
JO - Analytical Methods
JF - Analytical Methods
SN - 1759-9660
IS - 28
ER -
ID: 15959468