Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review
Trityl radicals as spin labels. / Krumkacheva, Olesya; Bagryanskaya, Elena.
Electron Paramagnetic ResonanceL: Volume 25. ed. / Chechik; DM Murphy. Royal Society of Chemistry, 2017. p. 35-60 (Electron Paramagnetic Resonance; Vol. 25).Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review
}
TY - CHAP
T1 - Trityl radicals as spin labels
AU - Krumkacheva, Olesya
AU - Bagryanskaya, Elena
PY - 2017/1/1
Y1 - 2017/1/1
N2 - During the last 30 years, tetrathiatriarylmethyl (TAM) radicals have been widely used as spin probes for oxymetry in electron paramagnetic resonance (EPR) spectroscopy and EPR tomography because of the narrow EPR linewidth and high stability in living systems. Recently, some researchers proposed to use TAMs as spin labels for studies on the structure of proteins and nucleic acids using site-directed spin labeling (SDSL) and pulsed dipolar EPR spectroscopy. In this chapter, the peculiarities of applying TAMs as spin labels, including the synthesis of TAMs, approaches to TAM spin labeling of proteins and nucleic acids, the methods for distance measurement using TAM spin labels, electron spin relaxation time, room temperature measurements, and advantages of orthogonal spin labeling are reviewed. Examples of applications of TAMs to research on the structure and functions of biopolymers are presented from recently published papers.
AB - During the last 30 years, tetrathiatriarylmethyl (TAM) radicals have been widely used as spin probes for oxymetry in electron paramagnetic resonance (EPR) spectroscopy and EPR tomography because of the narrow EPR linewidth and high stability in living systems. Recently, some researchers proposed to use TAMs as spin labels for studies on the structure of proteins and nucleic acids using site-directed spin labeling (SDSL) and pulsed dipolar EPR spectroscopy. In this chapter, the peculiarities of applying TAMs as spin labels, including the synthesis of TAMs, approaches to TAM spin labeling of proteins and nucleic acids, the methods for distance measurement using TAM spin labels, electron spin relaxation time, room temperature measurements, and advantages of orthogonal spin labeling are reviewed. Examples of applications of TAMs to research on the structure and functions of biopolymers are presented from recently published papers.
KW - ELECTRON-PARAMAGNETIC-RESONANCE
KW - INTRACELLULAR OXYGEN PROBES
KW - PULSED DIPOLAR EPR
KW - DISTANCE MEASUREMENTS
KW - TRIARYLMETHYL RADICALS
KW - ROOM-TEMPERATURE
KW - PHYSIOLOGICAL TEMPERATURES
KW - RELAXATION-ENHANCEMENT
KW - MAGNETIC-RESONANCE
KW - TISSUE OXYGEN
UR - http://www.scopus.com/inward/record.url?scp=85006508377&partnerID=8YFLogxK
U2 - 10.1039/9781782629436-00035
DO - 10.1039/9781782629436-00035
M3 - Chapter
AN - SCOPUS:85006508377
SN - 978-1-78262-857-6
T3 - Electron Paramagnetic Resonance
SP - 35
EP - 60
BT - Electron Paramagnetic ResonanceL
A2 - Chechik, null
A2 - Murphy, DM
PB - Royal Society of Chemistry
ER -
ID: 10318101