TY - CHAP

T1 - Trityl radicals as spin labels

AU - Krumkacheva, Olesya

AU - Bagryanskaya, Elena

PY - 2017/1/1

Y1 - 2017/1/1

N2 - During the last 30 years, tetrathiatriarylmethyl (TAM) radicals have been widely used as spin probes for oxymetry in electron paramagnetic resonance (EPR) spectroscopy and EPR tomography because of the narrow EPR linewidth and high stability in living systems. Recently, some researchers proposed to use TAMs as spin labels for studies on the structure of proteins and nucleic acids using site-directed spin labeling (SDSL) and pulsed dipolar EPR spectroscopy. In this chapter, the peculiarities of applying TAMs as spin labels, including the synthesis of TAMs, approaches to TAM spin labeling of proteins and nucleic acids, the methods for distance measurement using TAM spin labels, electron spin relaxation time, room temperature measurements, and advantages of orthogonal spin labeling are reviewed. Examples of applications of TAMs to research on the structure and functions of biopolymers are presented from recently published papers.

AB - During the last 30 years, tetrathiatriarylmethyl (TAM) radicals have been widely used as spin probes for oxymetry in electron paramagnetic resonance (EPR) spectroscopy and EPR tomography because of the narrow EPR linewidth and high stability in living systems. Recently, some researchers proposed to use TAMs as spin labels for studies on the structure of proteins and nucleic acids using site-directed spin labeling (SDSL) and pulsed dipolar EPR spectroscopy. In this chapter, the peculiarities of applying TAMs as spin labels, including the synthesis of TAMs, approaches to TAM spin labeling of proteins and nucleic acids, the methods for distance measurement using TAM spin labels, electron spin relaxation time, room temperature measurements, and advantages of orthogonal spin labeling are reviewed. Examples of applications of TAMs to research on the structure and functions of biopolymers are presented from recently published papers.

KW - ELECTRON-PARAMAGNETIC-RESONANCE

KW - INTRACELLULAR OXYGEN PROBES

KW - PULSED DIPOLAR EPR

KW - DISTANCE MEASUREMENTS

KW - TRIARYLMETHYL RADICALS

KW - ROOM-TEMPERATURE

KW - PHYSIOLOGICAL TEMPERATURES

KW - RELAXATION-ENHANCEMENT

KW - MAGNETIC-RESONANCE

KW - TISSUE OXYGEN

UR - http://www.scopus.com/inward/record.url?scp=85006508377&partnerID=8YFLogxK

U2 - 10.1039/9781782629436-00035

DO - 10.1039/9781782629436-00035

M3 - Chapter

AN - SCOPUS:85006508377

SN - 978-1-78262-857-6

T3 - Electron Paramagnetic Resonance

SP - 35

EP - 60

BT - Electron Paramagnetic ResonanceL

A2 - Chechik, null

A2 - Murphy, DM

PB - Royal Society of Chemistry

ER -