TY - JOUR

T1 - The phylogeny of class b flavoprotein monooxygenases and the origin of the yucca protein family

AU - Turnaev, Igor I.

AU - Gunbin, Konstantin V.

AU - Suslov, Valentin V.

AU - Akberdin, Ilya R.

AU - Kolchanov, Nikolay A.

AU - Afonnikov, Dmitry A.

N1 - Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/9/1

Y1 - 2020/9/1

N2 - YUCCA (YUCCA flavin-dependent monooxygenase) is one of the two enzymes of the main auxin biosynthesis pathway (tryptophan aminotransferase enzyme (TAA)/YUCCA) in land plants. The evolutionary origin of the YUCCA family is currently controversial: YUCCAs are assumed to have emerged via a horizontal gene transfer (HGT) from bacteria to the most recent common ancestor (MRCA) of land plants or to have inherited it from their ancestor, the charophyte algae. To refine YUCCA origin, we performed a phylogenetic analysis of the class B flavoprotein monooxygenases and comparative analysis of the sequences belonging to different families of this protein class. We distinguished a new protein family, named type IIb flavin-containing monooxygenases (FMOs), which comprises homologs of YUCCA from Rhodophyta, Chlorophyta, and Charophyta, land plant proteins, and FMO-E,-F, and-G of the bacterium Rhodococcus jostii RHA1. The type IIb FMOs differ considerably in the sites and domain composition from the other families of class B flavoprotein monooxygenases, YUCCAs included. The phylogenetic analysis also demonstrated that the type IIb FMO clade is not a sibling clade of YUCCAs. We have also identified the bacterial protein group named YUC-like FMOs as the closest to YUCCA homologs. Our results support the hypothesis of the emergence of YUCCA via HGT from bacteria to MRCA of land plants.

AB - YUCCA (YUCCA flavin-dependent monooxygenase) is one of the two enzymes of the main auxin biosynthesis pathway (tryptophan aminotransferase enzyme (TAA)/YUCCA) in land plants. The evolutionary origin of the YUCCA family is currently controversial: YUCCAs are assumed to have emerged via a horizontal gene transfer (HGT) from bacteria to the most recent common ancestor (MRCA) of land plants or to have inherited it from their ancestor, the charophyte algae. To refine YUCCA origin, we performed a phylogenetic analysis of the class B flavoprotein monooxygenases and comparative analysis of the sequences belonging to different families of this protein class. We distinguished a new protein family, named type IIb flavin-containing monooxygenases (FMOs), which comprises homologs of YUCCA from Rhodophyta, Chlorophyta, and Charophyta, land plant proteins, and FMO-E,-F, and-G of the bacterium Rhodococcus jostii RHA1. The type IIb FMOs differ considerably in the sites and domain composition from the other families of class B flavoprotein monooxygenases, YUCCAs included. The phylogenetic analysis also demonstrated that the type IIb FMO clade is not a sibling clade of YUCCAs. We have also identified the bacterial protein group named YUC-like FMOs as the closest to YUCCA homologs. Our results support the hypothesis of the emergence of YUCCA via HGT from bacteria to MRCA of land plants.

KW - Auxin biosynthesis

KW - Charophytes

KW - Flavin-dependent monooxygenase

KW - Horizontal gene transfer

KW - Phylogeny

KW - YUCCA

KW - charophytes

KW - horizontal gene transfer

KW - flavin-dependent monooxygenase

KW - MODES

KW - auxin biosynthesis

KW - EVOLUTION

KW - ENZYMES

KW - SEQUENCE

KW - ALGAE

KW - phylogeny

KW - FLAVIN-CONTAINING MONOOXYGENASES

KW - PLANT AUXIN BIOSYNTHESIS

KW - ARABIDOPSIS

UR - http://www.scopus.com/inward/record.url?scp=85090607952&partnerID=8YFLogxK

U2 - 10.3390/plants9091092

DO - 10.3390/plants9091092

M3 - Article

C2 - 32854417

AN - SCOPUS:85090607952

VL - 9

SP - 1

EP - 22

JO - Plants

JF - Plants

SN - 2223-7747

IS - 9

M1 - 1092

ER -