Research output: Contribution to journal › Article › peer-review
Specification and regulation of vascular tissue identity in the Arabidopsis embryo. / Smit, Margot E.; Llavata-Peris, Cristina I.; Roosjen, Mark et al.
In: Development (Cambridge), Vol. 147, No. 8, dev186130, 20.04.2020.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Specification and regulation of vascular tissue identity in the Arabidopsis embryo
AU - Smit, Margot E.
AU - Llavata-Peris, Cristina I.
AU - Roosjen, Mark
AU - van Beijnum, Henriette
AU - Novikova, Daria
AU - Levitsky, Victor
AU - Sevilem, Iris
AU - Roszak, Pawel
AU - Slane, Daniel
AU - Jürgens, Gerd
AU - Mironova, Victoria
AU - Brady, Siobhan M.
AU - Weijers, Dolf
N1 - © 2020. Published by The Company of Biologists Ltd.
PY - 2020/4/20
Y1 - 2020/4/20
N2 - Development of plant vascular tissues involves tissue identity specification, growth, pattern formation and cell-type differentiation. Although later developmental steps are understood in some detail, it is still largely unknown how the tissue is initially specified. We used the early Arabidopsis embryo as a simple model to study this process. Using a large collection of marker genes, we found that vascular identity was specified in the 16-cell embryo. After a transient precursor state, however, there was no persistent uniform tissue identity. Auxin is intimately connected to vascular tissue development. We found that, although an AUXIN RESPONSE FACTOR5/MONOPTEROS (ARF5/MP)-dependent auxin response was required, it was not sufficient for tissue specification. We therefore used a large-scale enhanced yeast one-hybrid assay to identify potential regulators of vascular identity. Network and functional analysis of candidate regulators suggest that vascular identity is under robust, complex control. We found that one candidate regulator, the G-class bZIP transcription factor GBF2, can modulate vascular gene expression by tuning MP output through direct interaction. Our work uncovers components of a gene regulatory network that controls the initial specification of vascular tissue identity.
AB - Development of plant vascular tissues involves tissue identity specification, growth, pattern formation and cell-type differentiation. Although later developmental steps are understood in some detail, it is still largely unknown how the tissue is initially specified. We used the early Arabidopsis embryo as a simple model to study this process. Using a large collection of marker genes, we found that vascular identity was specified in the 16-cell embryo. After a transient precursor state, however, there was no persistent uniform tissue identity. Auxin is intimately connected to vascular tissue development. We found that, although an AUXIN RESPONSE FACTOR5/MONOPTEROS (ARF5/MP)-dependent auxin response was required, it was not sufficient for tissue specification. We therefore used a large-scale enhanced yeast one-hybrid assay to identify potential regulators of vascular identity. Network and functional analysis of candidate regulators suggest that vascular identity is under robust, complex control. We found that one candidate regulator, the G-class bZIP transcription factor GBF2, can modulate vascular gene expression by tuning MP output through direct interaction. Our work uncovers components of a gene regulatory network that controls the initial specification of vascular tissue identity.
KW - Arabidopsis
KW - Auxin
KW - Embryo
KW - Gene regulatory network
KW - Vascular development
KW - ONE-HYBRID ASSAYS
KW - PROTEIN
KW - CELL FATE
KW - DNA-BINDING ACTIVITY
KW - BZIP TRANSCRIPTION FACTORS
KW - LIGATION-INDEPENDENT CLONING
KW - GENE FAMILY
KW - AUXIN-RESPONSE
KW - DIFFERENTIATION
KW - PRIMARY ROOT
UR - http://www.scopus.com/inward/record.url?scp=85083731351&partnerID=8YFLogxK
U2 - 10.1242/dev.186130
DO - 10.1242/dev.186130
M3 - Article
C2 - 32198154
AN - SCOPUS:85083731351
VL - 147
JO - Development (Cambridge)
JF - Development (Cambridge)
SN - 0950-1991
IS - 8
M1 - dev186130
ER -
ID: 24092993