TY - JOUR

T1 - Specification and regulation of vascular tissue identity in the Arabidopsis embryo

AU - Smit, Margot E.

AU - Llavata-Peris, Cristina I.

AU - Roosjen, Mark

AU - van Beijnum, Henriette

AU - Novikova, Daria

AU - Levitsky, Victor

AU - Sevilem, Iris

AU - Roszak, Pawel

AU - Slane, Daniel

AU - Jürgens, Gerd

AU - Mironova, Victoria

AU - Brady, Siobhan M.

AU - Weijers, Dolf

N1 - © 2020. Published by The Company of Biologists Ltd.

PY - 2020/4/20

Y1 - 2020/4/20

N2 - Development of plant vascular tissues involves tissue identity specification, growth, pattern formation and cell-type differentiation. Although later developmental steps are understood in some detail, it is still largely unknown how the tissue is initially specified. We used the early Arabidopsis embryo as a simple model to study this process. Using a large collection of marker genes, we found that vascular identity was specified in the 16-cell embryo. After a transient precursor state, however, there was no persistent uniform tissue identity. Auxin is intimately connected to vascular tissue development. We found that, although an AUXIN RESPONSE FACTOR5/MONOPTEROS (ARF5/MP)-dependent auxin response was required, it was not sufficient for tissue specification. We therefore used a large-scale enhanced yeast one-hybrid assay to identify potential regulators of vascular identity. Network and functional analysis of candidate regulators suggest that vascular identity is under robust, complex control. We found that one candidate regulator, the G-class bZIP transcription factor GBF2, can modulate vascular gene expression by tuning MP output through direct interaction. Our work uncovers components of a gene regulatory network that controls the initial specification of vascular tissue identity.

AB - Development of plant vascular tissues involves tissue identity specification, growth, pattern formation and cell-type differentiation. Although later developmental steps are understood in some detail, it is still largely unknown how the tissue is initially specified. We used the early Arabidopsis embryo as a simple model to study this process. Using a large collection of marker genes, we found that vascular identity was specified in the 16-cell embryo. After a transient precursor state, however, there was no persistent uniform tissue identity. Auxin is intimately connected to vascular tissue development. We found that, although an AUXIN RESPONSE FACTOR5/MONOPTEROS (ARF5/MP)-dependent auxin response was required, it was not sufficient for tissue specification. We therefore used a large-scale enhanced yeast one-hybrid assay to identify potential regulators of vascular identity. Network and functional analysis of candidate regulators suggest that vascular identity is under robust, complex control. We found that one candidate regulator, the G-class bZIP transcription factor GBF2, can modulate vascular gene expression by tuning MP output through direct interaction. Our work uncovers components of a gene regulatory network that controls the initial specification of vascular tissue identity.

KW - Arabidopsis

KW - Auxin

KW - Embryo

KW - Gene regulatory network

KW - Vascular development

KW - ONE-HYBRID ASSAYS

KW - PROTEIN

KW - CELL FATE

KW - DNA-BINDING ACTIVITY

KW - BZIP TRANSCRIPTION FACTORS

KW - LIGATION-INDEPENDENT CLONING

KW - GENE FAMILY

KW - AUXIN-RESPONSE

KW - DIFFERENTIATION

KW - PRIMARY ROOT

UR - http://www.scopus.com/inward/record.url?scp=85083731351&partnerID=8YFLogxK

U2 - 10.1242/dev.186130

DO - 10.1242/dev.186130

M3 - Article

C2 - 32198154

AN - SCOPUS:85083731351

VL - 147

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 8

M1 - dev186130

ER -