Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization

Standard

Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization. / Zairov, Rustem R.; Solovieva, Anastasiya O.; Shamsutdinova, Nataliya A. et al.

In: Materials Science and Engineering C, Vol. 95, 01.02.2019, p. 166-173.

Research output: Contribution to journal › Article › peer-review

Harvard

Zairov, RR, Solovieva, AO, Shamsutdinova, NA, Podyachev, SN, Shestopalov, MA, Pozmogova, TN, Miroshnichenko, SM, Mustafina, AR & Karasik, AA 2019, 'Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization', Materials Science and Engineering C, vol. 95, pp. 166-173. https://doi.org/10.1016/j.msec.2018.10.084

APA

Zairov, R. R., Solovieva, A. O., Shamsutdinova, N. A., Podyachev, S. N., Shestopalov, M. A., Pozmogova, T. N., Miroshnichenko, S. M., Mustafina, A. R., & Karasik, A. A. (2019). Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization. Materials Science and Engineering C, 95, 166-173. https://doi.org/10.1016/j.msec.2018.10.084

Vancouver

Zairov RR, Solovieva AO, Shamsutdinova NA, Podyachev SN, Shestopalov MA, Pozmogova TN et al. Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization. Materials Science and Engineering C. 2019 Feb 1;95:166-173. doi: 10.1016/j.msec.2018.10.084

Author

Zairov, Rustem R. ; Solovieva, Anastasiya O. ; Shamsutdinova, Nataliya A. et al. / Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization. In: Materials Science and Engineering C. 2019 ; Vol. 95. pp. 166-173.

BibTeX

@article{05a3c931a6d241b693a10e9a46a8de10,

title = "Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization",

abstract = "The present work reports ultra-small polyelectrolyte-coated water insoluble Tb(III) complex species with bright Tb(III)-centered luminescence resulted from efficient ligand-to-metal energy transfer as efficient labels for Hep-2 cells. The flow cytometry data revealed the enhanced cellular uptake of negatively charged nanoparticles coated by the polystyrenesulfonate (PSS)-monolayer versus the positively charged nanoparticles. The latter are obtained by layer-by-layer deposition of polyethyleneimine (PEI) onto PSS-coated ones. Confocal and TEM images of Hep-2 cells exposed by the colloids confirm favorable cell internalization of the PSS- compared to PEI-PSS-coated colloids illustrating unusual charge-effect. Dynamic light scattering data indicate significant effect of the biological background exemplified by serum bovine albumin and phosphatidylcholine-based bilayers on the exterior charge and aggregation behavior of the colloids. The obtained results reveal the PSS-coated nanoparticles based on water insoluble Tb(III) complex as promising cell labels.",

keywords = "Cell label, Cellular uptake, Layer-by-layer, Polyelectrolyte, Tb(III) chelate nanoparticles, Microscopy, Electron, Transmission, Humans, Luminescence, Hep G2 Cells, Microscopy, Confocal, Flow Cytometry, Nanoparticles/chemistry, Cell Line, Tumor, Polyelectrolytes/chemistry",

author = "Zairov, {Rustem R.} and Solovieva, {Anastasiya O.} and Shamsutdinova, {Nataliya A.} and Podyachev, {Sergey N.} and Shestopalov, {Michael A.} and Pozmogova, {Tatiana N.} and Miroshnichenko, {Svetlana M.} and Mustafina, {Asiya R.} and Karasik, {Andrey A.}",

year = "2019",

month = feb,

day = "1",

doi = "10.1016/j.msec.2018.10.084",

language = "English",

volume = "95",

pages = "166--173",

journal = "Materials Science and Engineering C",

issn = "0928-4931",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization

AU - Zairov, Rustem R.

AU - Solovieva, Anastasiya O.

AU - Shamsutdinova, Nataliya A.

AU - Podyachev, Sergey N.

AU - Shestopalov, Michael A.

AU - Pozmogova, Tatiana N.

AU - Miroshnichenko, Svetlana M.

AU - Mustafina, Asiya R.

AU - Karasik, Andrey A.

PY - 2019/2/1

Y1 - 2019/2/1

N2 - The present work reports ultra-small polyelectrolyte-coated water insoluble Tb(III) complex species with bright Tb(III)-centered luminescence resulted from efficient ligand-to-metal energy transfer as efficient labels for Hep-2 cells. The flow cytometry data revealed the enhanced cellular uptake of negatively charged nanoparticles coated by the polystyrenesulfonate (PSS)-monolayer versus the positively charged nanoparticles. The latter are obtained by layer-by-layer deposition of polyethyleneimine (PEI) onto PSS-coated ones. Confocal and TEM images of Hep-2 cells exposed by the colloids confirm favorable cell internalization of the PSS- compared to PEI-PSS-coated colloids illustrating unusual charge-effect. Dynamic light scattering data indicate significant effect of the biological background exemplified by serum bovine albumin and phosphatidylcholine-based bilayers on the exterior charge and aggregation behavior of the colloids. The obtained results reveal the PSS-coated nanoparticles based on water insoluble Tb(III) complex as promising cell labels.

AB - The present work reports ultra-small polyelectrolyte-coated water insoluble Tb(III) complex species with bright Tb(III)-centered luminescence resulted from efficient ligand-to-metal energy transfer as efficient labels for Hep-2 cells. The flow cytometry data revealed the enhanced cellular uptake of negatively charged nanoparticles coated by the polystyrenesulfonate (PSS)-monolayer versus the positively charged nanoparticles. The latter are obtained by layer-by-layer deposition of polyethyleneimine (PEI) onto PSS-coated ones. Confocal and TEM images of Hep-2 cells exposed by the colloids confirm favorable cell internalization of the PSS- compared to PEI-PSS-coated colloids illustrating unusual charge-effect. Dynamic light scattering data indicate significant effect of the biological background exemplified by serum bovine albumin and phosphatidylcholine-based bilayers on the exterior charge and aggregation behavior of the colloids. The obtained results reveal the PSS-coated nanoparticles based on water insoluble Tb(III) complex as promising cell labels.

KW - Cell label

KW - Cellular uptake

KW - Layer-by-layer

KW - Polyelectrolyte

KW - Tb(III) chelate nanoparticles

KW - Microscopy, Electron, Transmission

KW - Humans

KW - Luminescence

KW - Hep G2 Cells

KW - Microscopy, Confocal

KW - Flow Cytometry

KW - Nanoparticles/chemistry

KW - Cell Line, Tumor

KW - Polyelectrolytes/chemistry

UR - http://www.scopus.com/inward/record.url?scp=85055583626&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2018.10.084

DO - 10.1016/j.msec.2018.10.084

M3 - Article

C2 - 30573238

AN - SCOPUS:85055583626

VL - 95

SP - 166

EP - 173

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

ER -

ID: 17246919