Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization. / Zairov, Rustem R.; Solovieva, Anastasiya O.; Shamsutdinova, Nataliya A. и др.
в: Materials Science and Engineering C, Том 95, 01.02.2019, стр. 166-173.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization
AU - Zairov, Rustem R.
AU - Solovieva, Anastasiya O.
AU - Shamsutdinova, Nataliya A.
AU - Podyachev, Sergey N.
AU - Shestopalov, Michael A.
AU - Pozmogova, Tatiana N.
AU - Miroshnichenko, Svetlana M.
AU - Mustafina, Asiya R.
AU - Karasik, Andrey A.
N1 - Copyright © 2018 Elsevier B.V. All rights reserved.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - The present work reports ultra-small polyelectrolyte-coated water insoluble Tb(III) complex species with bright Tb(III)-centered luminescence resulted from efficient ligand-to-metal energy transfer as efficient labels for Hep-2 cells. The flow cytometry data revealed the enhanced cellular uptake of negatively charged nanoparticles coated by the polystyrenesulfonate (PSS)-monolayer versus the positively charged nanoparticles. The latter are obtained by layer-by-layer deposition of polyethyleneimine (PEI) onto PSS-coated ones. Confocal and TEM images of Hep-2 cells exposed by the colloids confirm favorable cell internalization of the PSS- compared to PEI-PSS-coated colloids illustrating unusual charge-effect. Dynamic light scattering data indicate significant effect of the biological background exemplified by serum bovine albumin and phosphatidylcholine-based bilayers on the exterior charge and aggregation behavior of the colloids. The obtained results reveal the PSS-coated nanoparticles based on water insoluble Tb(III) complex as promising cell labels.
AB - The present work reports ultra-small polyelectrolyte-coated water insoluble Tb(III) complex species with bright Tb(III)-centered luminescence resulted from efficient ligand-to-metal energy transfer as efficient labels for Hep-2 cells. The flow cytometry data revealed the enhanced cellular uptake of negatively charged nanoparticles coated by the polystyrenesulfonate (PSS)-monolayer versus the positively charged nanoparticles. The latter are obtained by layer-by-layer deposition of polyethyleneimine (PEI) onto PSS-coated ones. Confocal and TEM images of Hep-2 cells exposed by the colloids confirm favorable cell internalization of the PSS- compared to PEI-PSS-coated colloids illustrating unusual charge-effect. Dynamic light scattering data indicate significant effect of the biological background exemplified by serum bovine albumin and phosphatidylcholine-based bilayers on the exterior charge and aggregation behavior of the colloids. The obtained results reveal the PSS-coated nanoparticles based on water insoluble Tb(III) complex as promising cell labels.
KW - Cell label
KW - Cellular uptake
KW - Layer-by-layer
KW - Polyelectrolyte
KW - Tb(III) chelate nanoparticles
KW - Microscopy, Electron, Transmission
KW - Humans
KW - Luminescence
KW - Hep G2 Cells
KW - Microscopy, Confocal
KW - Flow Cytometry
KW - Nanoparticles/chemistry
KW - Cell Line, Tumor
KW - Polyelectrolytes/chemistry
UR - http://www.scopus.com/inward/record.url?scp=85055583626&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2018.10.084
DO - 10.1016/j.msec.2018.10.084
M3 - Article
C2 - 30573238
AN - SCOPUS:85055583626
VL - 95
SP - 166
EP - 173
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -
ID: 17246919