Research output: Contribution to journal › Article › peer-review
Luminescent silica mesoparticles for protein transduction. / Vorotnikov, Yuri A.; Pozmogova, Tatiana N.; Solovieva, Anastasiya O. et al.
In: Materials Science and Engineering C, Vol. 96, 01.03.2019, p. 530-538.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Luminescent silica mesoparticles for protein transduction
AU - Vorotnikov, Yuri A.
AU - Pozmogova, Tatiana N.
AU - Solovieva, Anastasiya O.
AU - Miroshnichenko, Svetlana M.
AU - Vorontsova, Elena V.
AU - Shestopalova, Lidiya V.
AU - Mironov, Yuri V.
AU - Shestopalov, Michael A.
AU - Efremova, Olga A.
N1 - Publisher Copyright: © 2018 Elsevier B.V.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Unlike silica nanoparticles, the potential of silica mesoparticles (SMPs) (i.e. particles of submicron size) for biological applications in particular the in vitro (let alone in vivo) cellular delivery of biological cargo has so far not been sufficiently studied. Here we examine the potential of luminescent (namely, octahedral molybdenum cluster doped) SMPs synthesised by a simple one-pot reaction for the labelling of cells and for protein transduction into larynx carcinoma (Hep-2) cells using GFP as a model protein. Our data demonstrates that the SMPs internalise into the cells within half an hour. This results in cells that detectably luminesce via conventional methods. In addition, the particles are non-toxic both in darkness and upon photo-irradiation. The SMPs were modified to allow their functionalisation by a protein, which then delivered the protein (GFP) efficiently into the cells. Thus, the luminescent SMPs offer a cheap and trackable alternative to existing materials for cellular internalisation of proteins, such as the HIV TAT protein and commercial protein delivery agents (e.g. Pierce™).
AB - Unlike silica nanoparticles, the potential of silica mesoparticles (SMPs) (i.e. particles of submicron size) for biological applications in particular the in vitro (let alone in vivo) cellular delivery of biological cargo has so far not been sufficiently studied. Here we examine the potential of luminescent (namely, octahedral molybdenum cluster doped) SMPs synthesised by a simple one-pot reaction for the labelling of cells and for protein transduction into larynx carcinoma (Hep-2) cells using GFP as a model protein. Our data demonstrates that the SMPs internalise into the cells within half an hour. This results in cells that detectably luminesce via conventional methods. In addition, the particles are non-toxic both in darkness and upon photo-irradiation. The SMPs were modified to allow their functionalisation by a protein, which then delivered the protein (GFP) efficiently into the cells. Thus, the luminescent SMPs offer a cheap and trackable alternative to existing materials for cellular internalisation of proteins, such as the HIV TAT protein and commercial protein delivery agents (e.g. Pierce™).
KW - Cellular uptake
KW - Cytotoxicity
KW - Octahedral molybdenum cluster
KW - Protein transduction
KW - Silica
KW - BIOCOMPATIBILITY
KW - MICROSPHERES
KW - PARTICLES
KW - MICROPARTICLES
KW - SIZE-DEPENDENT CYTOTOXICITY
KW - DELIVERY
KW - NANOPARTICLES
KW - IN-VITRO
KW - INTERNALIZATION
KW - CELL
KW - Silicon Dioxide/chemistry
KW - Humans
KW - Luminescence
KW - Green Fluorescent Proteins/chemistry
KW - Nanoparticles/chemistry
KW - Cell Line, Tumor
UR - http://www.scopus.com/inward/record.url?scp=85057779934&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2018.11.056
DO - 10.1016/j.msec.2018.11.056
M3 - Article
C2 - 30606563
AN - SCOPUS:85057779934
VL - 96
SP - 530
EP - 538
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -
ID: 17822399