Standard

Luminescent silica mesoparticles for protein transduction. / Vorotnikov, Yuri A.; Pozmogova, Tatiana N.; Solovieva, Anastasiya O. и др.

в: Materials Science and Engineering C, Том 96, 01.03.2019, стр. 530-538.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Vorotnikov, YA, Pozmogova, TN, Solovieva, AO, Miroshnichenko, SM, Vorontsova, EV, Shestopalova, LV, Mironov, YV, Shestopalov, MA & Efremova, OA 2019, 'Luminescent silica mesoparticles for protein transduction', Materials Science and Engineering C, Том. 96, стр. 530-538. https://doi.org/10.1016/j.msec.2018.11.056

APA

Vorotnikov, Y. A., Pozmogova, T. N., Solovieva, A. O., Miroshnichenko, S. M., Vorontsova, E. V., Shestopalova, L. V., Mironov, Y. V., Shestopalov, M. A., & Efremova, O. A. (2019). Luminescent silica mesoparticles for protein transduction. Materials Science and Engineering C, 96, 530-538. https://doi.org/10.1016/j.msec.2018.11.056

Vancouver

Vorotnikov YA, Pozmogova TN, Solovieva AO, Miroshnichenko SM, Vorontsova EV, Shestopalova LV и др. Luminescent silica mesoparticles for protein transduction. Materials Science and Engineering C. 2019 март 1;96:530-538. doi: 10.1016/j.msec.2018.11.056

Author

Vorotnikov, Yuri A. ; Pozmogova, Tatiana N. ; Solovieva, Anastasiya O. и др. / Luminescent silica mesoparticles for protein transduction. в: Materials Science and Engineering C. 2019 ; Том 96. стр. 530-538.

BibTeX

@article{b9e420abb0904d51b294c804a2f74955,
title = "Luminescent silica mesoparticles for protein transduction",
abstract = "Unlike silica nanoparticles, the potential of silica mesoparticles (SMPs) (i.e. particles of submicron size) for biological applications in particular the in vitro (let alone in vivo) cellular delivery of biological cargo has so far not been sufficiently studied. Here we examine the potential of luminescent (namely, octahedral molybdenum cluster doped) SMPs synthesised by a simple one-pot reaction for the labelling of cells and for protein transduction into larynx carcinoma (Hep-2) cells using GFP as a model protein. Our data demonstrates that the SMPs internalise into the cells within half an hour. This results in cells that detectably luminesce via conventional methods. In addition, the particles are non-toxic both in darkness and upon photo-irradiation. The SMPs were modified to allow their functionalisation by a protein, which then delivered the protein (GFP) efficiently into the cells. Thus, the luminescent SMPs offer a cheap and trackable alternative to existing materials for cellular internalisation of proteins, such as the HIV TAT protein and commercial protein delivery agents (e.g. Pierce{\texttrademark}).",
keywords = "Cellular uptake, Cytotoxicity, Octahedral molybdenum cluster, Protein transduction, Silica, BIOCOMPATIBILITY, MICROSPHERES, PARTICLES, MICROPARTICLES, SIZE-DEPENDENT CYTOTOXICITY, DELIVERY, NANOPARTICLES, IN-VITRO, INTERNALIZATION, CELL, Silicon Dioxide/chemistry, Humans, Luminescence, Green Fluorescent Proteins/chemistry, Nanoparticles/chemistry, Cell Line, Tumor",
author = "Vorotnikov, {Yuri A.} and Pozmogova, {Tatiana N.} and Solovieva, {Anastasiya O.} and Miroshnichenko, {Svetlana M.} and Vorontsova, {Elena V.} and Shestopalova, {Lidiya V.} and Mironov, {Yuri V.} and Shestopalov, {Michael A.} and Efremova, {Olga A.}",
note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",
year = "2019",
month = mar,
day = "1",
doi = "10.1016/j.msec.2018.11.056",
language = "English",
volume = "96",
pages = "530--538",
journal = "Materials Science and Engineering C",
issn = "0928-4931",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Luminescent silica mesoparticles for protein transduction

AU - Vorotnikov, Yuri A.

AU - Pozmogova, Tatiana N.

AU - Solovieva, Anastasiya O.

AU - Miroshnichenko, Svetlana M.

AU - Vorontsova, Elena V.

AU - Shestopalova, Lidiya V.

AU - Mironov, Yuri V.

AU - Shestopalov, Michael A.

AU - Efremova, Olga A.

N1 - Publisher Copyright: © 2018 Elsevier B.V.

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Unlike silica nanoparticles, the potential of silica mesoparticles (SMPs) (i.e. particles of submicron size) for biological applications in particular the in vitro (let alone in vivo) cellular delivery of biological cargo has so far not been sufficiently studied. Here we examine the potential of luminescent (namely, octahedral molybdenum cluster doped) SMPs synthesised by a simple one-pot reaction for the labelling of cells and for protein transduction into larynx carcinoma (Hep-2) cells using GFP as a model protein. Our data demonstrates that the SMPs internalise into the cells within half an hour. This results in cells that detectably luminesce via conventional methods. In addition, the particles are non-toxic both in darkness and upon photo-irradiation. The SMPs were modified to allow their functionalisation by a protein, which then delivered the protein (GFP) efficiently into the cells. Thus, the luminescent SMPs offer a cheap and trackable alternative to existing materials for cellular internalisation of proteins, such as the HIV TAT protein and commercial protein delivery agents (e.g. Pierce™).

AB - Unlike silica nanoparticles, the potential of silica mesoparticles (SMPs) (i.e. particles of submicron size) for biological applications in particular the in vitro (let alone in vivo) cellular delivery of biological cargo has so far not been sufficiently studied. Here we examine the potential of luminescent (namely, octahedral molybdenum cluster doped) SMPs synthesised by a simple one-pot reaction for the labelling of cells and for protein transduction into larynx carcinoma (Hep-2) cells using GFP as a model protein. Our data demonstrates that the SMPs internalise into the cells within half an hour. This results in cells that detectably luminesce via conventional methods. In addition, the particles are non-toxic both in darkness and upon photo-irradiation. The SMPs were modified to allow their functionalisation by a protein, which then delivered the protein (GFP) efficiently into the cells. Thus, the luminescent SMPs offer a cheap and trackable alternative to existing materials for cellular internalisation of proteins, such as the HIV TAT protein and commercial protein delivery agents (e.g. Pierce™).

KW - Cellular uptake

KW - Cytotoxicity

KW - Octahedral molybdenum cluster

KW - Protein transduction

KW - Silica

KW - BIOCOMPATIBILITY

KW - MICROSPHERES

KW - PARTICLES

KW - MICROPARTICLES

KW - SIZE-DEPENDENT CYTOTOXICITY

KW - DELIVERY

KW - NANOPARTICLES

KW - IN-VITRO

KW - INTERNALIZATION

KW - CELL

KW - Silicon Dioxide/chemistry

KW - Humans

KW - Luminescence

KW - Green Fluorescent Proteins/chemistry

KW - Nanoparticles/chemistry

KW - Cell Line, Tumor

UR - http://www.scopus.com/inward/record.url?scp=85057779934&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2018.11.056

DO - 10.1016/j.msec.2018.11.056

M3 - Article

C2 - 30606563

AN - SCOPUS:85057779934

VL - 96

SP - 530

EP - 538

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

ER -

ID: 17822399