Research output: Contribution to journal › Article › peer-review
Immunocytological Analysis of Meiotic Recombination in the Gray Goose (Anser anser). / Torgasheva, Anna A.; Borodin, Pavel M.
In: Cytogenetic and Genome Research, Vol. 151, No. 1, 01.04.2017, p. 27-35.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Immunocytological Analysis of Meiotic Recombination in the Gray Goose (Anser anser)
AU - Torgasheva, Anna A.
AU - Borodin, Pavel M.
N1 - Publisher Copyright: © 2017 S. Karger AG, Basel.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Studies on mammals demonstrate wide interspecific variation in the number and distribution of recombination events along chromosomes. Birds represent an interesting model group for comparative analysis of cytological and ecological drivers of recombination rate evolution. Yet, data on variation in recombination rates in birds are limited to a dozen of species. In this study, we used immunolocalization of MLH1, a mismatch repair protein marking mature recombination nodules, to estimate the overall recombination rate and distribution of crossovers along macrochromosomes in female and male meiosis of the gray goose (Anser anser). The average number of MLH1 foci was significantly higher in oocytes than in spermatocytes (73.6 ± 7.8 and 58.9 ± 7.6, respectively). MLH1 foci distribution along individual macrobivalents showed subtelomeric peaks, which were more pronounced in males. Analysis of distances between neighboring MLH1 foci on macrobivalents revealed stronger crossover interference in male meiosis. These data create a framework for future genetic and physical mapping of the gray goose.
AB - Studies on mammals demonstrate wide interspecific variation in the number and distribution of recombination events along chromosomes. Birds represent an interesting model group for comparative analysis of cytological and ecological drivers of recombination rate evolution. Yet, data on variation in recombination rates in birds are limited to a dozen of species. In this study, we used immunolocalization of MLH1, a mismatch repair protein marking mature recombination nodules, to estimate the overall recombination rate and distribution of crossovers along macrochromosomes in female and male meiosis of the gray goose (Anser anser). The average number of MLH1 foci was significantly higher in oocytes than in spermatocytes (73.6 ± 7.8 and 58.9 ± 7.6, respectively). MLH1 foci distribution along individual macrobivalents showed subtelomeric peaks, which were more pronounced in males. Analysis of distances between neighboring MLH1 foci on macrobivalents revealed stronger crossover interference in male meiosis. These data create a framework for future genetic and physical mapping of the gray goose.
KW - Avian chromosomes
KW - Crossover distribution
KW - Crossover interference
KW - Meiosis
KW - MLH1
KW - SYCP3
KW - Synaptonemal complex
KW - Immunohistochemistry
KW - Meiosis/genetics
KW - Homologous Recombination
KW - Male
KW - MutL Protein Homolog 1/metabolism
KW - Female
KW - Chromosomes/genetics
KW - Spermatocytes/metabolism
KW - Chromosome Segregation
KW - Crossing Over, Genetic
KW - Pachytene Stage
KW - Geese/genetics
KW - Synaptonemal Complex
KW - Avian Proteins/metabolism
KW - Animals
KW - Oocytes/metabolism
KW - Chromosome Pairing
KW - Karyotype
KW - EQUAL RECOMBINATION
KW - SYNAPTONEMAL COMPLEX LENGTH
KW - CHICKEN
KW - GENETIC-LINKAGE MAPS
KW - AVIAN GENOME EVOLUTION
KW - CHROMOSOME
KW - CROSSING-OVER
KW - FICEDULA-ALBICOLLIS
KW - REVEALS
UR - http://www.scopus.com/inward/record.url?scp=85015653874&partnerID=8YFLogxK
U2 - 10.1159/000458741
DO - 10.1159/000458741
M3 - Article
C2 - 28297694
AN - SCOPUS:85015653874
VL - 151
SP - 27
EP - 35
JO - Cytogenetic and Genome Research
JF - Cytogenetic and Genome Research
SN - 1424-8581
IS - 1
ER -
ID: 8975401