TY - JOUR

T1 - Immunocytological Analysis of Meiotic Recombination in the Gray Goose (Anser anser)

AU - Torgasheva, Anna A.

AU - Borodin, Pavel M.

N1 - Publisher Copyright: © 2017 S. Karger AG, Basel.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Studies on mammals demonstrate wide interspecific variation in the number and distribution of recombination events along chromosomes. Birds represent an interesting model group for comparative analysis of cytological and ecological drivers of recombination rate evolution. Yet, data on variation in recombination rates in birds are limited to a dozen of species. In this study, we used immunolocalization of MLH1, a mismatch repair protein marking mature recombination nodules, to estimate the overall recombination rate and distribution of crossovers along macrochromosomes in female and male meiosis of the gray goose (Anser anser). The average number of MLH1 foci was significantly higher in oocytes than in spermatocytes (73.6 ± 7.8 and 58.9 ± 7.6, respectively). MLH1 foci distribution along individual macrobivalents showed subtelomeric peaks, which were more pronounced in males. Analysis of distances between neighboring MLH1 foci on macrobivalents revealed stronger crossover interference in male meiosis. These data create a framework for future genetic and physical mapping of the gray goose.

AB - Studies on mammals demonstrate wide interspecific variation in the number and distribution of recombination events along chromosomes. Birds represent an interesting model group for comparative analysis of cytological and ecological drivers of recombination rate evolution. Yet, data on variation in recombination rates in birds are limited to a dozen of species. In this study, we used immunolocalization of MLH1, a mismatch repair protein marking mature recombination nodules, to estimate the overall recombination rate and distribution of crossovers along macrochromosomes in female and male meiosis of the gray goose (Anser anser). The average number of MLH1 foci was significantly higher in oocytes than in spermatocytes (73.6 ± 7.8 and 58.9 ± 7.6, respectively). MLH1 foci distribution along individual macrobivalents showed subtelomeric peaks, which were more pronounced in males. Analysis of distances between neighboring MLH1 foci on macrobivalents revealed stronger crossover interference in male meiosis. These data create a framework for future genetic and physical mapping of the gray goose.

KW - Avian chromosomes

KW - Crossover distribution

KW - Crossover interference

KW - Meiosis

KW - MLH1

KW - SYCP3

KW - Synaptonemal complex

KW - Immunohistochemistry

KW - Meiosis/genetics

KW - Homologous Recombination

KW - Male

KW - MutL Protein Homolog 1/metabolism

KW - Female

KW - Chromosomes/genetics

KW - Spermatocytes/metabolism

KW - Chromosome Segregation

KW - Crossing Over, Genetic

KW - Pachytene Stage

KW - Geese/genetics

KW - Synaptonemal Complex

KW - Avian Proteins/metabolism

KW - Animals

KW - Oocytes/metabolism

KW - Chromosome Pairing

KW - Karyotype

KW - EQUAL RECOMBINATION

KW - SYNAPTONEMAL COMPLEX LENGTH

KW - CHICKEN

KW - GENETIC-LINKAGE MAPS

KW - AVIAN GENOME EVOLUTION

KW - CHROMOSOME

KW - CROSSING-OVER

KW - FICEDULA-ALBICOLLIS

KW - REVEALS

UR - http://www.scopus.com/inward/record.url?scp=85015653874&partnerID=8YFLogxK

U2 - 10.1159/000458741

DO - 10.1159/000458741

M3 - Article

C2 - 28297694

AN - SCOPUS:85015653874

VL - 151

SP - 27

EP - 35

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

IS - 1

ER -