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Genes containing long introns occupy series of bands and interbands in drosophila melanogaster polytene chromosomes. / Khoroshko, Varvara A.; Pokholkova, Galina V.; Levitsky, Victor G. et al.
In: Genes, Vol. 11, No. 4, 417, 04.2020.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Genes containing long introns occupy series of bands and interbands in drosophila melanogaster polytene chromosomes
AU - Khoroshko, Varvara A.
AU - Pokholkova, Galina V.
AU - Levitsky, Victor G.
AU - Zykova, Tatyana Yu
AU - Antonenko, Oksana V.
AU - Belyaeva, Elena S.
AU - Zhimulev, Igor F.
PY - 2020/4
Y1 - 2020/4
N2 - The Drosophila melanogaster polytene chromosomes are the best model for studying the genome organization during interphase. Despite of the long-term studies available on genetic organization of polytene chromosome bands and interbands, little is known regarding long gene location on chromosomes. To analyze it, we used bioinformatic approaches and characterized genome-wide distribution of introns in gene bodies and in different chromatin states, and using fluorescent in situ hybridization we juxtaposed them with the chromosome structures. Short introns up to 2 kb in length are located in the bodies of housekeeping genes (grey bands or lazurite chromatin). In the group of 70 longest genes in the Drosophila genome, 95% of total gene length accrues to introns. The mapping of the 15 long genes showed that they could occupy extended sections of polytene chromosomes containing band and interband series, with promoters located in the interband fragments (aquamarine chromatin). Introns (malachite and ruby chromatin) in polytene chromosomes form independent bands, which can contain either both introns and exons or intron material only. Thus, a novel type of the gene arrangement in polytene chromosomes was discovered; peculiarities of such genetic organization are discussed.
AB - The Drosophila melanogaster polytene chromosomes are the best model for studying the genome organization during interphase. Despite of the long-term studies available on genetic organization of polytene chromosome bands and interbands, little is known regarding long gene location on chromosomes. To analyze it, we used bioinformatic approaches and characterized genome-wide distribution of introns in gene bodies and in different chromatin states, and using fluorescent in situ hybridization we juxtaposed them with the chromosome structures. Short introns up to 2 kb in length are located in the bodies of housekeeping genes (grey bands or lazurite chromatin). In the group of 70 longest genes in the Drosophila genome, 95% of total gene length accrues to introns. The mapping of the 15 long genes showed that they could occupy extended sections of polytene chromosomes containing band and interband series, with promoters located in the interband fragments (aquamarine chromatin). Introns (malachite and ruby chromatin) in polytene chromosomes form independent bands, which can contain either both introns and exons or intron material only. Thus, a novel type of the gene arrangement in polytene chromosomes was discovered; peculiarities of such genetic organization are discussed.
KW - Bands
KW - Chromatin states
KW - Drosophila melanogaster
KW - Gene introns
KW - Housekeeping genes
KW - Interbands
KW - Polytene chromosomes
KW - interbands
KW - X-CHROMOSOME
KW - SEQUENCES
KW - gene introns
KW - chromatin states
KW - bands
KW - 2B3-4-2B11
KW - CYTOGENETIC ANALYSIS
KW - REGION
KW - polytene chromosomes
KW - REPLICATION
KW - DNA
KW - FUNCTIONAL-ORGANIZATION
KW - REVISED MAP
KW - METAMORPHOSIS
KW - housekeeping genes
UR - http://www.scopus.com/inward/record.url?scp=85083376040&partnerID=8YFLogxK
U2 - 10.3390/genes11040417
DO - 10.3390/genes11040417
M3 - Article
C2 - 32290448
AN - SCOPUS:85083376040
VL - 11
JO - Genes
JF - Genes
SN - 2073-4425
IS - 4
M1 - 417
ER -
ID: 24092825