Research output: Contribution to journal › Article › peer-review
Enrichment of Grain Anthocyanin Content through Marker-Assisted Breeding for Ant1, Ant2 or HvMyc2 Genes in Barley (Hordeum vulgare L.). / Kukoeva, Tatjana V.; Molobekova, Camilla A.; Totsky, Igor V. et al.
In: Agronomy, Vol. 14, No. 6, 1231, 06.2024.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Enrichment of Grain Anthocyanin Content through Marker-Assisted Breeding for Ant1, Ant2 or HvMyc2 Genes in Barley (Hordeum vulgare L.)
AU - Kukoeva, Tatjana V.
AU - Molobekova, Camilla A.
AU - Totsky, Igor V.
AU - Vasiliev, Gennady V.
AU - Pronozin, Artem Yu
AU - Afonnikov, Dmitry A.
AU - Khlestkina, Elena K.
AU - Shoeva, Olesya Yu
N1 - This research was funded by the Russian Science Foundation, grant number 21-76-10024.
PY - 2024/6
Y1 - 2024/6
N2 - Breeding of cereals with anthocyanin-rich grains is promising for health food production. Here, we transferred genes responsible for activation of anthocyanin biosynthesis in the grain pericarp (Ant1 and Ant2) and aleurone (HvMyc2) to elite barley cultivars Aley, Tanay, and Vorsinsky-2 by marker-assisted backcrossing. Donors were Bowman lines BW648 and BW418. Three pairs of near-isogenic lines (NILs) with purple or blue colored grains were chosen in generation BC6F2 and propagated up to BC6F6. Genotyping-by-sequencing of resulting NILs and parental lines showed that the NILs carry 4.6–17.6% of donor SNPs including those at target loci. Unexpected big blocks of donor SNPs were revealed in some NILs on chromosomes 1H and 5H that do not carry target loci. The NILs possessed 274% and 12% higher anthocyanin (40.0–170.4 μg/g) and total phenolic content (2367.2–4396.8 μg/g), respectively, compared to original cultivars (18.3–33.1 μg/g and 2319.6–3768.5 μg/g). On average, antioxidant activity was 14% higher, although some lines showed reduced activity. NILs’ productivity depended on growth conditions and was similar to that of the recurrent cultivars. Hence, the applied breeding strategy is an effective approach to enrichment of grain anthocyanin content in barley.
AB - Breeding of cereals with anthocyanin-rich grains is promising for health food production. Here, we transferred genes responsible for activation of anthocyanin biosynthesis in the grain pericarp (Ant1 and Ant2) and aleurone (HvMyc2) to elite barley cultivars Aley, Tanay, and Vorsinsky-2 by marker-assisted backcrossing. Donors were Bowman lines BW648 and BW418. Three pairs of near-isogenic lines (NILs) with purple or blue colored grains were chosen in generation BC6F2 and propagated up to BC6F6. Genotyping-by-sequencing of resulting NILs and parental lines showed that the NILs carry 4.6–17.6% of donor SNPs including those at target loci. Unexpected big blocks of donor SNPs were revealed in some NILs on chromosomes 1H and 5H that do not carry target loci. The NILs possessed 274% and 12% higher anthocyanin (40.0–170.4 μg/g) and total phenolic content (2367.2–4396.8 μg/g), respectively, compared to original cultivars (18.3–33.1 μg/g and 2319.6–3768.5 μg/g). On average, antioxidant activity was 14% higher, although some lines showed reduced activity. NILs’ productivity depended on growth conditions and was similar to that of the recurrent cultivars. Hence, the applied breeding strategy is an effective approach to enrichment of grain anthocyanin content in barley.
KW - DPPH
KW - Hordeum vulgareL
KW - flavonoids
KW - functional food
KW - grain quality
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85197197530&origin=inward&txGid=4524dbb56c965cf2764f0844893977a6
UR - https://www.mendeley.com/catalogue/080faf73-fce7-3486-a914-e80278b95fd9/
U2 - 10.3390/agronomy14061231
DO - 10.3390/agronomy14061231
M3 - Article
VL - 14
JO - Agronomy
JF - Agronomy
SN - 2073-4395
IS - 6
M1 - 1231
ER -
ID: 60750123