TY - JOUR

T1 - Enrichment of Grain Anthocyanin Content through Marker-Assisted Breeding for Ant1, Ant2 or HvMyc2 Genes in Barley (Hordeum vulgare L.)

AU - Kukoeva, Tatjana V.

AU - Molobekova, Camilla A.

AU - Totsky, Igor V.

AU - Vasiliev, Gennady V.

AU - Pronozin, Artem Yu

AU - Afonnikov, Dmitry A.

AU - Khlestkina, Elena K.

AU - Shoeva, Olesya Yu

N1 - This research was funded by the Russian Science Foundation, grant number 21-76-10024.

PY - 2024/6

Y1 - 2024/6

N2 - Breeding of cereals with anthocyanin-rich grains is promising for health food production. Here, we transferred genes responsible for activation of anthocyanin biosynthesis in the grain pericarp (Ant1 and Ant2) and aleurone (HvMyc2) to elite barley cultivars Aley, Tanay, and Vorsinsky-2 by marker-assisted backcrossing. Donors were Bowman lines BW648 and BW418. Three pairs of near-isogenic lines (NILs) with purple or blue colored grains were chosen in generation BC6F2 and propagated up to BC6F6. Genotyping-by-sequencing of resulting NILs and parental lines showed that the NILs carry 4.6–17.6% of donor SNPs including those at target loci. Unexpected big blocks of donor SNPs were revealed in some NILs on chromosomes 1H and 5H that do not carry target loci. The NILs possessed 274% and 12% higher anthocyanin (40.0–170.4 μg/g) and total phenolic content (2367.2–4396.8 μg/g), respectively, compared to original cultivars (18.3–33.1 μg/g and 2319.6–3768.5 μg/g). On average, antioxidant activity was 14% higher, although some lines showed reduced activity. NILs’ productivity depended on growth conditions and was similar to that of the recurrent cultivars. Hence, the applied breeding strategy is an effective approach to enrichment of grain anthocyanin content in barley.

AB - Breeding of cereals with anthocyanin-rich grains is promising for health food production. Here, we transferred genes responsible for activation of anthocyanin biosynthesis in the grain pericarp (Ant1 and Ant2) and aleurone (HvMyc2) to elite barley cultivars Aley, Tanay, and Vorsinsky-2 by marker-assisted backcrossing. Donors were Bowman lines BW648 and BW418. Three pairs of near-isogenic lines (NILs) with purple or blue colored grains were chosen in generation BC6F2 and propagated up to BC6F6. Genotyping-by-sequencing of resulting NILs and parental lines showed that the NILs carry 4.6–17.6% of donor SNPs including those at target loci. Unexpected big blocks of donor SNPs were revealed in some NILs on chromosomes 1H and 5H that do not carry target loci. The NILs possessed 274% and 12% higher anthocyanin (40.0–170.4 μg/g) and total phenolic content (2367.2–4396.8 μg/g), respectively, compared to original cultivars (18.3–33.1 μg/g and 2319.6–3768.5 μg/g). On average, antioxidant activity was 14% higher, although some lines showed reduced activity. NILs’ productivity depended on growth conditions and was similar to that of the recurrent cultivars. Hence, the applied breeding strategy is an effective approach to enrichment of grain anthocyanin content in barley.

KW - DPPH

KW - Hordeum vulgareL

KW - flavonoids

KW - functional food

KW - grain quality

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85197197530&origin=inward&txGid=4524dbb56c965cf2764f0844893977a6

UR - https://www.mendeley.com/catalogue/080faf73-fce7-3486-a914-e80278b95fd9/

U2 - 10.3390/agronomy14061231

DO - 10.3390/agronomy14061231

M3 - Article

VL - 14

JO - Agronomy

JF - Agronomy

SN - 2073-4395

IS - 6

M1 - 1231

ER -