Research output: Contribution to journal › Article › peer-review
Cell Dynamics in WOX5-Overexpressing Root Tips : The Impact of Local Auxin Biosynthesis. / Savina, Maria S.; Pasternak, Taras; Omelyanchuk, Nadya A. et al.
In: Frontiers in Plant Science, Vol. 11, 560169, 22.10.2020.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Cell Dynamics in WOX5-Overexpressing Root Tips
T2 - The Impact of Local Auxin Biosynthesis
AU - Savina, Maria S.
AU - Pasternak, Taras
AU - Omelyanchuk, Nadya A.
AU - Novikova, Daria D.
AU - Palme, Klaus
AU - Mironova, Victoria V.
AU - Lavrekha, Viktoriya V.
N1 - Publisher Copyright: © Copyright © 2020 Savina, Pasternak, Omelyanchuk, Novikova, Palme, Mironova and Lavrekha. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10/22
Y1 - 2020/10/22
N2 - Root stem cell niche functioning requires the formation and maintenance of the specific “auxin-rich domain” governed by directional auxin transport and local auxin production. Auxin maximum co-localizes with the WOX5 expression domain in the quiescent center that separates mitotically active proximal and distal root meristems. Here we unravel the interconnected processes happening under WOX5 overexpression by combining in vivo experiments and mathematical modeling. We showed that WOX5-induced TAA1-mediated auxin biosynthesis is the cause, whereas auxin accumulation, PIN transporters relocation, and auxin redistribution between proximal and distal root meristems are its subsequent effects that influence the formation of the well-described phenotype with an enlarged root cap. These findings helped us to clarify the role of WOX5, which serves as a local QC-specific regulator that activates biosynthesis of non-cell-autonomous signal auxin to regulate the distal meristem functioning. The mathematical model with WOX5-mediated auxin biosynthesis and auxin-regulated cell growth, division, and detachment reproduces the columella cells dynamics in both wild type and under WOX5 dysregulation.
AB - Root stem cell niche functioning requires the formation and maintenance of the specific “auxin-rich domain” governed by directional auxin transport and local auxin production. Auxin maximum co-localizes with the WOX5 expression domain in the quiescent center that separates mitotically active proximal and distal root meristems. Here we unravel the interconnected processes happening under WOX5 overexpression by combining in vivo experiments and mathematical modeling. We showed that WOX5-induced TAA1-mediated auxin biosynthesis is the cause, whereas auxin accumulation, PIN transporters relocation, and auxin redistribution between proximal and distal root meristems are its subsequent effects that influence the formation of the well-described phenotype with an enlarged root cap. These findings helped us to clarify the role of WOX5, which serves as a local QC-specific regulator that activates biosynthesis of non-cell-autonomous signal auxin to regulate the distal meristem functioning. The mathematical model with WOX5-mediated auxin biosynthesis and auxin-regulated cell growth, division, and detachment reproduces the columella cells dynamics in both wild type and under WOX5 dysregulation.
KW - auxin
KW - EdU
KW - image analysis
KW - iRoCS toolbox
KW - mathematical model
KW - mitotic activity
KW - RAM
KW - WOX5
KW - SIGNAL
KW - APICAL MERISTEM
KW - SHOOT
KW - ORGANIZATION
KW - GROWTH
KW - ARABIDOPSIS
KW - DIFFERENTIATION
UR - http://www.scopus.com/inward/record.url?scp=85095682742&partnerID=8YFLogxK
U2 - 10.3389/fpls.2020.560169
DO - 10.3389/fpls.2020.560169
M3 - Article
C2 - 33193486
AN - SCOPUS:85095682742
VL - 11
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 560169
ER -
ID: 25997930