TY - JOUR

T1 - Cell Dynamics in WOX5-Overexpressing Root Tips

T2 - The Impact of Local Auxin Biosynthesis

AU - Savina, Maria S.

AU - Pasternak, Taras

AU - Omelyanchuk, Nadya A.

AU - Novikova, Daria D.

AU - Palme, Klaus

AU - Mironova, Victoria V.

AU - Lavrekha, Viktoriya V.

N1 - Publisher Copyright: © Copyright © 2020 Savina, Pasternak, Omelyanchuk, Novikova, Palme, Mironova and Lavrekha. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/10/22

Y1 - 2020/10/22

N2 - Root stem cell niche functioning requires the formation and maintenance of the specific “auxin-rich domain” governed by directional auxin transport and local auxin production. Auxin maximum co-localizes with the WOX5 expression domain in the quiescent center that separates mitotically active proximal and distal root meristems. Here we unravel the interconnected processes happening under WOX5 overexpression by combining in vivo experiments and mathematical modeling. We showed that WOX5-induced TAA1-mediated auxin biosynthesis is the cause, whereas auxin accumulation, PIN transporters relocation, and auxin redistribution between proximal and distal root meristems are its subsequent effects that influence the formation of the well-described phenotype with an enlarged root cap. These findings helped us to clarify the role of WOX5, which serves as a local QC-specific regulator that activates biosynthesis of non-cell-autonomous signal auxin to regulate the distal meristem functioning. The mathematical model with WOX5-mediated auxin biosynthesis and auxin-regulated cell growth, division, and detachment reproduces the columella cells dynamics in both wild type and under WOX5 dysregulation.

AB - Root stem cell niche functioning requires the formation and maintenance of the specific “auxin-rich domain” governed by directional auxin transport and local auxin production. Auxin maximum co-localizes with the WOX5 expression domain in the quiescent center that separates mitotically active proximal and distal root meristems. Here we unravel the interconnected processes happening under WOX5 overexpression by combining in vivo experiments and mathematical modeling. We showed that WOX5-induced TAA1-mediated auxin biosynthesis is the cause, whereas auxin accumulation, PIN transporters relocation, and auxin redistribution between proximal and distal root meristems are its subsequent effects that influence the formation of the well-described phenotype with an enlarged root cap. These findings helped us to clarify the role of WOX5, which serves as a local QC-specific regulator that activates biosynthesis of non-cell-autonomous signal auxin to regulate the distal meristem functioning. The mathematical model with WOX5-mediated auxin biosynthesis and auxin-regulated cell growth, division, and detachment reproduces the columella cells dynamics in both wild type and under WOX5 dysregulation.

KW - auxin

KW - EdU

KW - image analysis

KW - iRoCS toolbox

KW - mathematical model

KW - mitotic activity

KW - RAM

KW - WOX5

KW - SIGNAL

KW - APICAL MERISTEM

KW - SHOOT

KW - ORGANIZATION

KW - GROWTH

KW - ARABIDOPSIS

KW - DIFFERENTIATION

UR - http://www.scopus.com/inward/record.url?scp=85095682742&partnerID=8YFLogxK

U2 - 10.3389/fpls.2020.560169

DO - 10.3389/fpls.2020.560169

M3 - Article

C2 - 33193486

AN - SCOPUS:85095682742

VL - 11

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 560169

ER -