TY - JOUR

T1 - Unique Wolbachia strain wMelPlus increases heat stress resistance in Drosophila melanogaster

AU - Burdina, Elena V.

AU - Bykov, Roman A.

AU - Menshanov, Petr N.

AU - Ilinsky, Yury Yu

AU - Gruntenko, Nataly

N1 - Funding Information: This study was supported by the State Budgeted Project #0259‐2021‐0016 and the RFBR #20‐04‐00579. We thank Darya Kochetova for language editing. Publisher Copyright: © 2021 Wiley Periodicals LLC Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/4

Y1 - 2021/4

N2 - Maternally inherited endosymbiotic bacterium Wolbachia infects Drosophila melanogaster populations worldwide. Its genetic diversity includes several closely related genotypes, which can be attributed to two main genotype groups: wMel and wMelCS. Here, we studied eight D. melanogaster lines carrying the nuclear background of wild type interbred Bi90 line and cytoplasmic backgrounds with or without Wolbachia of different origin, each of which belongs to wMelCS genotype group. We analyzed the effect these seven Wolbachia strains had on the heat stress resistance and dopamine metabolism in D. melanogaster females. Survival under heat stress (38°C, 3 h 30 min) was increased only in the line infected with bacteria of the wMelPlus strain. At the same time, the activity of alkaline phosphatase (an enzyme regulating the pool of dopamine precursor tyrosine) was increased under normal conditions in females infected with all strains under study and retained the response to heat stress typical for the uninfected line. Thus, we found the unique Wolbachia strain that provides an increase of the host stress resistance, and demonstrated that the mechanism of this resistance is not dopamine-mediated.

AB - Maternally inherited endosymbiotic bacterium Wolbachia infects Drosophila melanogaster populations worldwide. Its genetic diversity includes several closely related genotypes, which can be attributed to two main genotype groups: wMel and wMelCS. Here, we studied eight D. melanogaster lines carrying the nuclear background of wild type interbred Bi90 line and cytoplasmic backgrounds with or without Wolbachia of different origin, each of which belongs to wMelCS genotype group. We analyzed the effect these seven Wolbachia strains had on the heat stress resistance and dopamine metabolism in D. melanogaster females. Survival under heat stress (38°C, 3 h 30 min) was increased only in the line infected with bacteria of the wMelPlus strain. At the same time, the activity of alkaline phosphatase (an enzyme regulating the pool of dopamine precursor tyrosine) was increased under normal conditions in females infected with all strains under study and retained the response to heat stress typical for the uninfected line. Thus, we found the unique Wolbachia strain that provides an increase of the host stress resistance, and demonstrated that the mechanism of this resistance is not dopamine-mediated.

KW - alkaline phosphatase

KW - Drosophila

KW - heat stress

KW - stress resistance

KW - Wolbachia

KW - Wolbachia/genetics

KW - Genotype

KW - Symbiosis/physiology

KW - Drosophila melanogaster/metabolism

KW - Thermotolerance/genetics

KW - Heat-Shock Response

KW - Animals

KW - Dopamine/metabolism

KW - Alkaline Phosphatase/metabolism

UR - http://www.scopus.com/inward/record.url?scp=85101857097&partnerID=8YFLogxK

U2 - 10.1002/arch.21776

DO - 10.1002/arch.21776

M3 - Article

C2 - 33644932

AN - SCOPUS:85101857097

VL - 106

JO - Archives of Insect Biochemistry and Physiology

JF - Archives of Insect Biochemistry and Physiology

SN - 0739-4462

IS - 4

M1 - e21776

ER -