Research output: Contribution to journal › Article › peer-review
Unique Wolbachia strain wMelPlus increases heat stress resistance in Drosophila melanogaster. / Burdina, Elena V.; Bykov, Roman A.; Menshanov, Petr N. et al.
In: Archives of Insect Biochemistry and Physiology, Vol. 106, No. 4, e21776, 04.2021.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Unique Wolbachia strain wMelPlus increases heat stress resistance in Drosophila melanogaster
AU - Burdina, Elena V.
AU - Bykov, Roman A.
AU - Menshanov, Petr N.
AU - Ilinsky, Yury Yu
AU - Gruntenko, Nataly
N1 - Funding Information: This study was supported by the State Budgeted Project #0259‐2021‐0016 and the RFBR #20‐04‐00579. We thank Darya Kochetova for language editing. Publisher Copyright: © 2021 Wiley Periodicals LLC Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/4
Y1 - 2021/4
N2 - Maternally inherited endosymbiotic bacterium Wolbachia infects Drosophila melanogaster populations worldwide. Its genetic diversity includes several closely related genotypes, which can be attributed to two main genotype groups: wMel and wMelCS. Here, we studied eight D. melanogaster lines carrying the nuclear background of wild type interbred Bi90 line and cytoplasmic backgrounds with or without Wolbachia of different origin, each of which belongs to wMelCS genotype group. We analyzed the effect these seven Wolbachia strains had on the heat stress resistance and dopamine metabolism in D. melanogaster females. Survival under heat stress (38°C, 3 h 30 min) was increased only in the line infected with bacteria of the wMelPlus strain. At the same time, the activity of alkaline phosphatase (an enzyme regulating the pool of dopamine precursor tyrosine) was increased under normal conditions in females infected with all strains under study and retained the response to heat stress typical for the uninfected line. Thus, we found the unique Wolbachia strain that provides an increase of the host stress resistance, and demonstrated that the mechanism of this resistance is not dopamine-mediated.
AB - Maternally inherited endosymbiotic bacterium Wolbachia infects Drosophila melanogaster populations worldwide. Its genetic diversity includes several closely related genotypes, which can be attributed to two main genotype groups: wMel and wMelCS. Here, we studied eight D. melanogaster lines carrying the nuclear background of wild type interbred Bi90 line and cytoplasmic backgrounds with or without Wolbachia of different origin, each of which belongs to wMelCS genotype group. We analyzed the effect these seven Wolbachia strains had on the heat stress resistance and dopamine metabolism in D. melanogaster females. Survival under heat stress (38°C, 3 h 30 min) was increased only in the line infected with bacteria of the wMelPlus strain. At the same time, the activity of alkaline phosphatase (an enzyme regulating the pool of dopamine precursor tyrosine) was increased under normal conditions in females infected with all strains under study and retained the response to heat stress typical for the uninfected line. Thus, we found the unique Wolbachia strain that provides an increase of the host stress resistance, and demonstrated that the mechanism of this resistance is not dopamine-mediated.
KW - alkaline phosphatase
KW - Drosophila
KW - heat stress
KW - stress resistance
KW - Wolbachia
KW - Wolbachia/genetics
KW - Genotype
KW - Symbiosis/physiology
KW - Drosophila melanogaster/metabolism
KW - Thermotolerance/genetics
KW - Heat-Shock Response
KW - Animals
KW - Dopamine/metabolism
KW - Alkaline Phosphatase/metabolism
UR - http://www.scopus.com/inward/record.url?scp=85101857097&partnerID=8YFLogxK
U2 - 10.1002/arch.21776
DO - 10.1002/arch.21776
M3 - Article
C2 - 33644932
AN - SCOPUS:85101857097
VL - 106
JO - Archives of Insect Biochemistry and Physiology
JF - Archives of Insect Biochemistry and Physiology
SN - 0739-4462
IS - 4
M1 - e21776
ER -
ID: 28012898