TY - JOUR

T1 - Unilateral stimulation of the lateral division of the dorsal telencephalon induces synaptic plasticity in the bilateral medial division of zebrafish

AU - Wu, Yao Ju

AU - Chen, Yu Lan

AU - Tang, Tso Hao

AU - Ng, Ming Chong

AU - Amstislavskaya, Tamara G.

AU - Tikhonova, Maria A.

AU - Yang, Yi Ling

AU - Lu, Kwok Tung

PY - 2017/8/22

Y1 - 2017/8/22

N2 - This study was aimed to evaluate the synaptic plasticity in projections from the dorsal lateral region (Dl) to the bilateral dorsal medial region (Dm) of the zebrafish telencephalon. The results showed that unilateral electrical stimulation of the Dl evokes a negative field potential (FP) in both the contralateral and ipsilateral side of the Dm. We tested synaptic plasticity, including high-frequency stimulation-induced LTP (HFS-LTP) and low-frequency stimulation-induced LTD (LFS-LTD). We demonstrated that HFS-induced bilateral LTP is NMDAR-dependent by the application of an NMDAR antagonist, DL-AP5 (30 μM, suprafused for 10 min), which blocked the HFS-induced LTP in both the contralateral and ipsilateral Dm. In addition, LTP was restored after DL-AP5 was washed out by continuous aCSF suprafusion. These results suggested that the potentiation is NMDAR-dependent. Either LFS (1 Hz for 20 min) or applying the mGluR agonist, DHPG (40 μM, suprafused for 10 min) successfully induced bilateral LTD for at least 1 h. Furthermore, both the contralateral fEPSP and LTP vanished after ablation of the anterior commissure. In conclusion, the results of the present study suggested that the projection between the Dl and contralateral Dm in the telencephalon of zebrafish is via the anterior commissure and possesses synaptic plasticity.

AB - This study was aimed to evaluate the synaptic plasticity in projections from the dorsal lateral region (Dl) to the bilateral dorsal medial region (Dm) of the zebrafish telencephalon. The results showed that unilateral electrical stimulation of the Dl evokes a negative field potential (FP) in both the contralateral and ipsilateral side of the Dm. We tested synaptic plasticity, including high-frequency stimulation-induced LTP (HFS-LTP) and low-frequency stimulation-induced LTD (LFS-LTD). We demonstrated that HFS-induced bilateral LTP is NMDAR-dependent by the application of an NMDAR antagonist, DL-AP5 (30 μM, suprafused for 10 min), which blocked the HFS-induced LTP in both the contralateral and ipsilateral Dm. In addition, LTP was restored after DL-AP5 was washed out by continuous aCSF suprafusion. These results suggested that the potentiation is NMDAR-dependent. Either LFS (1 Hz for 20 min) or applying the mGluR agonist, DHPG (40 μM, suprafused for 10 min) successfully induced bilateral LTD for at least 1 h. Furthermore, both the contralateral fEPSP and LTP vanished after ablation of the anterior commissure. In conclusion, the results of the present study suggested that the projection between the Dl and contralateral Dm in the telencephalon of zebrafish is via the anterior commissure and possesses synaptic plasticity.

KW - Animals

KW - Electric Stimulation/methods

KW - Evoked Potentials

KW - Long-Term Potentiation

KW - Male

KW - Methoxyhydroxyphenylglycol/analogs & derivatives

KW - Neuronal Plasticity

KW - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors

KW - Telencephalon/physiology

KW - Zebrafish/physiology

KW - NMDA RECEPTORS

KW - CONNECTIONS

KW - AREA CA1

KW - HIPPOCAMPUS

KW - MEMORY

KW - CA1 REGION

KW - GOLDFISH

KW - IN-VIVO

KW - LONG-TERM POTENTIATION

KW - DHPG-INDUCED LTD

UR - http://www.scopus.com/inward/record.url?scp=85028025447&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-08093-9

DO - 10.1038/s41598-017-08093-9

M3 - Article

C2 - 28831099

AN - SCOPUS:85028025447

VL - 7

SP - 9096

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 9096

ER -