Unilateral stimulation of the lateral division of the dorsal telencephalon induces synaptic plasticity in the bilateral medial division of zebrafish. / Wu, Yao Ju; Chen, Yu Lan; Tang, Tso Hao et al.
In: Scientific Reports, Vol. 7, No. 1, 9096, 22.08.2017, p. 9096.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Unilateral stimulation of the lateral division of the dorsal telencephalon induces synaptic plasticity in the bilateral medial division of zebrafish
AU - Wu, Yao Ju
AU - Chen, Yu Lan
AU - Tang, Tso Hao
AU - Ng, Ming Chong
AU - Amstislavskaya, Tamara G.
AU - Tikhonova, Maria A.
AU - Yang, Yi Ling
AU - Lu, Kwok Tung
PY - 2017/8/22
Y1 - 2017/8/22
N2 - This study was aimed to evaluate the synaptic plasticity in projections from the dorsal lateral region (Dl) to the bilateral dorsal medial region (Dm) of the zebrafish telencephalon. The results showed that unilateral electrical stimulation of the Dl evokes a negative field potential (FP) in both the contralateral and ipsilateral side of the Dm. We tested synaptic plasticity, including high-frequency stimulation-induced LTP (HFS-LTP) and low-frequency stimulation-induced LTD (LFS-LTD). We demonstrated that HFS-induced bilateral LTP is NMDAR-dependent by the application of an NMDAR antagonist, DL-AP5 (30 μM, suprafused for 10 min), which blocked the HFS-induced LTP in both the contralateral and ipsilateral Dm. In addition, LTP was restored after DL-AP5 was washed out by continuous aCSF suprafusion. These results suggested that the potentiation is NMDAR-dependent. Either LFS (1 Hz for 20 min) or applying the mGluR agonist, DHPG (40 μM, suprafused for 10 min) successfully induced bilateral LTD for at least 1 h. Furthermore, both the contralateral fEPSP and LTP vanished after ablation of the anterior commissure. In conclusion, the results of the present study suggested that the projection between the Dl and contralateral Dm in the telencephalon of zebrafish is via the anterior commissure and possesses synaptic plasticity.
AB - This study was aimed to evaluate the synaptic plasticity in projections from the dorsal lateral region (Dl) to the bilateral dorsal medial region (Dm) of the zebrafish telencephalon. The results showed that unilateral electrical stimulation of the Dl evokes a negative field potential (FP) in both the contralateral and ipsilateral side of the Dm. We tested synaptic plasticity, including high-frequency stimulation-induced LTP (HFS-LTP) and low-frequency stimulation-induced LTD (LFS-LTD). We demonstrated that HFS-induced bilateral LTP is NMDAR-dependent by the application of an NMDAR antagonist, DL-AP5 (30 μM, suprafused for 10 min), which blocked the HFS-induced LTP in both the contralateral and ipsilateral Dm. In addition, LTP was restored after DL-AP5 was washed out by continuous aCSF suprafusion. These results suggested that the potentiation is NMDAR-dependent. Either LFS (1 Hz for 20 min) or applying the mGluR agonist, DHPG (40 μM, suprafused for 10 min) successfully induced bilateral LTD for at least 1 h. Furthermore, both the contralateral fEPSP and LTP vanished after ablation of the anterior commissure. In conclusion, the results of the present study suggested that the projection between the Dl and contralateral Dm in the telencephalon of zebrafish is via the anterior commissure and possesses synaptic plasticity.
KW - Animals
KW - Electric Stimulation/methods
KW - Evoked Potentials
KW - Long-Term Potentiation
KW - Male
KW - Methoxyhydroxyphenylglycol/analogs & derivatives
KW - Neuronal Plasticity
KW - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
KW - Telencephalon/physiology
KW - Zebrafish/physiology
KW - NMDA RECEPTORS
KW - CONNECTIONS
KW - AREA CA1
KW - HIPPOCAMPUS
KW - MEMORY
KW - CA1 REGION
KW - GOLDFISH
KW - IN-VIVO
KW - LONG-TERM POTENTIATION
KW - DHPG-INDUCED LTD
UR - http://www.scopus.com/inward/record.url?scp=85028025447&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-08093-9
DO - 10.1038/s41598-017-08093-9
M3 - Article
C2 - 28831099
AN - SCOPUS:85028025447
VL - 7
SP - 9096
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 9096
ER -
ID: 9444097