Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Triazine-Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells. / Sztandera, Krzysztof; Gorzkiewicz, Michał; Bątal, Mateusz и др.
в: International journal of nanomedicine, Том 17, 2022, стр. 1139-1154.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Triazine-Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells
AU - Sztandera, Krzysztof
AU - Gorzkiewicz, Michał
AU - Bątal, Mateusz
AU - Arkhipova, Valeria
AU - Knauer, Nadezhda
AU - Sánchez-Nieves, Javier
AU - de la Mata, Fco Javier
AU - Gómez, Rafael
AU - Apartsin, Evgeny
AU - Klajnert-Maculewicz, Barbara
N1 - Funding Information: The authors thank Dr. Julia Poletaeva and Prof. Elena Ryabchikova (ICBFM) for the TEM study of dendrimersomes and Dr. Ervin Epstein (Children’s Oakland Research Institute, Oakland, CA, USA) for providing murine basal cell carcinoma lines (AsZ, BsZ, CsZ). This work was supported by National Science Centre, Poland (Project UMO-2017/25/B/NZ7/ 01304 “Phosphorus dendrimers as carriers for photosensitizers - in vivo studies”), by RFBR grant No. 18-33-20109, and based upon work from COST Action CA17140 NANO2CLINIC “Cancer Nanomedicine - from the bench to the bedside” supported by COST (European Cooperation in Science and Technology). The project received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 844217. This work was also supported by PID2020-112924RB-I00 (Ministry of Science and Innovation), Consortium IMMUNOTHERCAN-CM B2017/BMD-3733 (CAM), NANODENDMED II-CM ref B2017/BMD-3703 and project SBPLY/17/180501/000358 JCCM and PIE14/00061 (CIBER-BBN). Publisher Copyright: © 2022 Sztandera et al.
PY - 2022
Y1 - 2022
N2 - Background: The search for new formulations for photodynamic therapy is intended to improve the outcome of skin cancer treatment using significantly reduced doses of photosensitizer, thereby avoiding side effects. The incorporation of photosensitizers into nanoassemblies is a versatile way to increase the efficiency and specificity of drug delivery into target cells. Herein, we report the loading of rose bengal into vesicle-like constructs of amphiphilic triazine-carbosilane dendrons (dendrimersomes) as well as biophysical and in vitro characterization of this novel nanosystem. Methods: Using established protocol and analytical and spectroscopy techniques we were able to synthesized dendrons with strictly designed properties. Engaging biophysical methods (hydrodynamic diameter and zeta potential measurements, analysis of spectral properties, transmission electron microscopy) we confirmed assembling of our nanosystem. A set of in vitro techniques was used for determination ROS generation, (ABDA and H2DCFDA probes), cell viability (MTT assay) and cellular uptake (flow cytometry and confocal microscopy). Results: Encapsulation of rose bengal inside dendrimersomes enhances cellular uptake, intracellular ROS production and concequently, the phototoxicity of this photosensitizer. Conclusion: Triazine-carbosilane dendrimersomes show high capacity as drug carriers for anticancer photodynamic therapy.
AB - Background: The search for new formulations for photodynamic therapy is intended to improve the outcome of skin cancer treatment using significantly reduced doses of photosensitizer, thereby avoiding side effects. The incorporation of photosensitizers into nanoassemblies is a versatile way to increase the efficiency and specificity of drug delivery into target cells. Herein, we report the loading of rose bengal into vesicle-like constructs of amphiphilic triazine-carbosilane dendrons (dendrimersomes) as well as biophysical and in vitro characterization of this novel nanosystem. Methods: Using established protocol and analytical and spectroscopy techniques we were able to synthesized dendrons with strictly designed properties. Engaging biophysical methods (hydrodynamic diameter and zeta potential measurements, analysis of spectral properties, transmission electron microscopy) we confirmed assembling of our nanosystem. A set of in vitro techniques was used for determination ROS generation, (ABDA and H2DCFDA probes), cell viability (MTT assay) and cellular uptake (flow cytometry and confocal microscopy). Results: Encapsulation of rose bengal inside dendrimersomes enhances cellular uptake, intracellular ROS production and concequently, the phototoxicity of this photosensitizer. Conclusion: Triazine-carbosilane dendrimersomes show high capacity as drug carriers for anticancer photodynamic therapy.
KW - amphiphiles
KW - carbosilane
KW - dendrimersomes
KW - dendrons
KW - photodynamic therapy
KW - rose bengal
KW - Triazines/pharmacology
KW - Carcinoma
KW - Humans
KW - Silanes/pharmacology
KW - Rose Bengal/chemistry
UR - http://www.scopus.com/inward/record.url?scp=85126864335&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=48193903
U2 - 10.2147/IJN.S352349
DO - 10.2147/IJN.S352349
M3 - Article
C2 - 35321027
AN - SCOPUS:85126864335
VL - 17
SP - 1139
EP - 1154
JO - International journal of nanomedicine
JF - International journal of nanomedicine
SN - 1176-9114
ER -
ID: 35769460