TY - JOUR

T1 - Triazine-Carbosilane Dendrimersomes Enhance Cellular Uptake and Phototoxic Activity of Rose Bengal in Basal Cell Skin Carcinoma Cells

AU - Sztandera, Krzysztof

AU - Gorzkiewicz, Michał

AU - Bątal, Mateusz

AU - Arkhipova, Valeria

AU - Knauer, Nadezhda

AU - Sánchez-Nieves, Javier

AU - de la Mata, Fco Javier

AU - Gómez, Rafael

AU - Apartsin, Evgeny

AU - Klajnert-Maculewicz, Barbara

N1 - Funding Information: The authors thank Dr. Julia Poletaeva and Prof. Elena Ryabchikova (ICBFM) for the TEM study of dendrimersomes and Dr. Ervin Epstein (Children’s Oakland Research Institute, Oakland, CA, USA) for providing murine basal cell carcinoma lines (AsZ, BsZ, CsZ). This work was supported by National Science Centre, Poland (Project UMO-2017/25/B/NZ7/ 01304 “Phosphorus dendrimers as carriers for photosensitizers - in vivo studies”), by RFBR grant No. 18-33-20109, and based upon work from COST Action CA17140 NANO2CLINIC “Cancer Nanomedicine - from the bench to the bedside” supported by COST (European Cooperation in Science and Technology). The project received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 844217. This work was also supported by PID2020-112924RB-I00 (Ministry of Science and Innovation), Consortium IMMUNOTHERCAN-CM B2017/BMD-3733 (CAM), NANODENDMED II-CM ref B2017/BMD-3703 and project SBPLY/17/180501/000358 JCCM and PIE14/00061 (CIBER-BBN). Publisher Copyright: © 2022 Sztandera et al.

PY - 2022

Y1 - 2022

N2 - Background: The search for new formulations for photodynamic therapy is intended to improve the outcome of skin cancer treatment using significantly reduced doses of photosensitizer, thereby avoiding side effects. The incorporation of photosensitizers into nanoassemblies is a versatile way to increase the efficiency and specificity of drug delivery into target cells. Herein, we report the loading of rose bengal into vesicle-like constructs of amphiphilic triazine-carbosilane dendrons (dendrimersomes) as well as biophysical and in vitro characterization of this novel nanosystem. Methods: Using established protocol and analytical and spectroscopy techniques we were able to synthesized dendrons with strictly designed properties. Engaging biophysical methods (hydrodynamic diameter and zeta potential measurements, analysis of spectral properties, transmission electron microscopy) we confirmed assembling of our nanosystem. A set of in vitro techniques was used for determination ROS generation, (ABDA and H2DCFDA probes), cell viability (MTT assay) and cellular uptake (flow cytometry and confocal microscopy). Results: Encapsulation of rose bengal inside dendrimersomes enhances cellular uptake, intracellular ROS production and concequently, the phototoxicity of this photosensitizer. Conclusion: Triazine-carbosilane dendrimersomes show high capacity as drug carriers for anticancer photodynamic therapy.

AB - Background: The search for new formulations for photodynamic therapy is intended to improve the outcome of skin cancer treatment using significantly reduced doses of photosensitizer, thereby avoiding side effects. The incorporation of photosensitizers into nanoassemblies is a versatile way to increase the efficiency and specificity of drug delivery into target cells. Herein, we report the loading of rose bengal into vesicle-like constructs of amphiphilic triazine-carbosilane dendrons (dendrimersomes) as well as biophysical and in vitro characterization of this novel nanosystem. Methods: Using established protocol and analytical and spectroscopy techniques we were able to synthesized dendrons with strictly designed properties. Engaging biophysical methods (hydrodynamic diameter and zeta potential measurements, analysis of spectral properties, transmission electron microscopy) we confirmed assembling of our nanosystem. A set of in vitro techniques was used for determination ROS generation, (ABDA and H2DCFDA probes), cell viability (MTT assay) and cellular uptake (flow cytometry and confocal microscopy). Results: Encapsulation of rose bengal inside dendrimersomes enhances cellular uptake, intracellular ROS production and concequently, the phototoxicity of this photosensitizer. Conclusion: Triazine-carbosilane dendrimersomes show high capacity as drug carriers for anticancer photodynamic therapy.

KW - amphiphiles

KW - carbosilane

KW - dendrimersomes

KW - dendrons

KW - photodynamic therapy

KW - rose bengal

KW - Triazines/pharmacology

KW - Carcinoma

KW - Humans

KW - Silanes/pharmacology

KW - Rose Bengal/chemistry

UR - http://www.scopus.com/inward/record.url?scp=85126864335&partnerID=8YFLogxK

UR - https://www.elibrary.ru/item.asp?id=48193903

U2 - 10.2147/IJN.S352349

DO - 10.2147/IJN.S352349

M3 - Article

C2 - 35321027

AN - SCOPUS:85126864335

VL - 17

SP - 1139

EP - 1154

JO - International journal of nanomedicine

JF - International journal of nanomedicine

SN - 1176-9114

ER -