TY - JOUR

T1 - The Viromes of Mosquitoes from the Natural Landscapes of Western Siberia

AU - Ternovoi, Vladimir A

AU - Shvalov, Alexander N

AU - Kartashov, Mikhail Yu

AU - Ponomareva, Eugenia P

AU - Tupota, Natalia L

AU - Khoroshavin, Yuri A

AU - Bayandin, Roman B

AU - Gladysheva, Anastasia V

AU - Mikryukova, Tamara P

AU - Tregubchak, Tatyana V

AU - Loktev, Valery B

N1 - This work was supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement No. 075-15-2019-1665) and as part of a state assignment (9/21) for the State Scientific Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance of Consumer Rights Protection and Human Welfare.

PY - 2023/9/8

Y1 - 2023/9/8

N2 - The metagenomic analysis of mosquitoes allows for the genetic characterization of mosquito-associated viruses in different regions of the world. This study applied a metagenomic approach to identify novel viral sequences in seven species of mosquitoes collected from the Novosibirsk region of western Siberia. Using NGS sequencing, we identified 15 coding-complete viral polyproteins (genomes) and 15 viral-like partial sequences in mosquitoes. The complete sequences for novel viruses or the partial sequences of capsid proteins, hypothetical viral proteins, and RdRps were used to identify their taxonomy. The novel viral sequences were classified within the orders Tymovirales and Picornavirales and the families Partitiviridae, Totiviridae, Tombusviridae, Iflaviridae, Nodaviridae, Permutotetraviridae, and Solemoviridae, with several attributed to four unclassified RNA viruses. Interestingly, the novel putative viruses and viral sequences were mainly associated with the mosquito Coquillettidia richardii. This study aimed to increase our understanding of the viral diversity in mosquitoes found in the natural habitats of Siberia, which is characterized by very long, snowy, and cold winters.

AB - The metagenomic analysis of mosquitoes allows for the genetic characterization of mosquito-associated viruses in different regions of the world. This study applied a metagenomic approach to identify novel viral sequences in seven species of mosquitoes collected from the Novosibirsk region of western Siberia. Using NGS sequencing, we identified 15 coding-complete viral polyproteins (genomes) and 15 viral-like partial sequences in mosquitoes. The complete sequences for novel viruses or the partial sequences of capsid proteins, hypothetical viral proteins, and RdRps were used to identify their taxonomy. The novel viral sequences were classified within the orders Tymovirales and Picornavirales and the families Partitiviridae, Totiviridae, Tombusviridae, Iflaviridae, Nodaviridae, Permutotetraviridae, and Solemoviridae, with several attributed to four unclassified RNA viruses. Interestingly, the novel putative viruses and viral sequences were mainly associated with the mosquito Coquillettidia richardii. This study aimed to increase our understanding of the viral diversity in mosquitoes found in the natural habitats of Siberia, which is characterized by very long, snowy, and cold winters.

KW - Humans

KW - Animals

KW - Virome

KW - Siberia

KW - Nodaviridae

KW - Capsid Proteins/genetics

KW - Culicidae

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85172481242&origin=inward&txGid=62d3388722dc9472777272ca67ff01ee

UR - https://www.mendeley.com/catalogue/e2686181-c70e-3d8d-9260-1c6c6c3e3493/

U2 - 10.3390/v15091896

DO - 10.3390/v15091896

M3 - Article

C2 - 37766302

VL - 15

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 9

M1 - 1896

ER -