Research output: Contribution to journal › Article › peer-review
The Viromes of Mosquitoes from the Natural Landscapes of Western Siberia. / Ternovoi, Vladimir A; Shvalov, Alexander N; Kartashov, Mikhail Yu et al.
In: Viruses, Vol. 15, No. 9, 1896, 08.09.2023.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The Viromes of Mosquitoes from the Natural Landscapes of Western Siberia
AU - Ternovoi, Vladimir A
AU - Shvalov, Alexander N
AU - Kartashov, Mikhail Yu
AU - Ponomareva, Eugenia P
AU - Tupota, Natalia L
AU - Khoroshavin, Yuri A
AU - Bayandin, Roman B
AU - Gladysheva, Anastasia V
AU - Mikryukova, Tamara P
AU - Tregubchak, Tatyana V
AU - Loktev, Valery B
N1 - This work was supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement No. 075-15-2019-1665) and as part of a state assignment (9/21) for the State Scientific Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance of Consumer Rights Protection and Human Welfare.
PY - 2023/9/8
Y1 - 2023/9/8
N2 - The metagenomic analysis of mosquitoes allows for the genetic characterization of mosquito-associated viruses in different regions of the world. This study applied a metagenomic approach to identify novel viral sequences in seven species of mosquitoes collected from the Novosibirsk region of western Siberia. Using NGS sequencing, we identified 15 coding-complete viral polyproteins (genomes) and 15 viral-like partial sequences in mosquitoes. The complete sequences for novel viruses or the partial sequences of capsid proteins, hypothetical viral proteins, and RdRps were used to identify their taxonomy. The novel viral sequences were classified within the orders Tymovirales and Picornavirales and the families Partitiviridae, Totiviridae, Tombusviridae, Iflaviridae, Nodaviridae, Permutotetraviridae, and Solemoviridae, with several attributed to four unclassified RNA viruses. Interestingly, the novel putative viruses and viral sequences were mainly associated with the mosquito Coquillettidia richardii. This study aimed to increase our understanding of the viral diversity in mosquitoes found in the natural habitats of Siberia, which is characterized by very long, snowy, and cold winters.
AB - The metagenomic analysis of mosquitoes allows for the genetic characterization of mosquito-associated viruses in different regions of the world. This study applied a metagenomic approach to identify novel viral sequences in seven species of mosquitoes collected from the Novosibirsk region of western Siberia. Using NGS sequencing, we identified 15 coding-complete viral polyproteins (genomes) and 15 viral-like partial sequences in mosquitoes. The complete sequences for novel viruses or the partial sequences of capsid proteins, hypothetical viral proteins, and RdRps were used to identify their taxonomy. The novel viral sequences were classified within the orders Tymovirales and Picornavirales and the families Partitiviridae, Totiviridae, Tombusviridae, Iflaviridae, Nodaviridae, Permutotetraviridae, and Solemoviridae, with several attributed to four unclassified RNA viruses. Interestingly, the novel putative viruses and viral sequences were mainly associated with the mosquito Coquillettidia richardii. This study aimed to increase our understanding of the viral diversity in mosquitoes found in the natural habitats of Siberia, which is characterized by very long, snowy, and cold winters.
KW - Humans
KW - Animals
KW - Virome
KW - Siberia
KW - Nodaviridae
KW - Capsid Proteins/genetics
KW - Culicidae
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85172481242&origin=inward&txGid=62d3388722dc9472777272ca67ff01ee
UR - https://www.mendeley.com/catalogue/e2686181-c70e-3d8d-9260-1c6c6c3e3493/
U2 - 10.3390/v15091896
DO - 10.3390/v15091896
M3 - Article
C2 - 37766302
VL - 15
JO - Viruses
JF - Viruses
SN - 1999-4915
IS - 9
M1 - 1896
ER -
ID: 56213097