Standard

The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice. / Kurilin, Vasiliy V.; Khantakova, Julia N.; Tereschenko, Valeriy P. и др.

в: Journal of Immunology Research, Том 2019, 7029726, 01.01.2019, стр. 7029726.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Kurilin, VV, Khantakova, JN, Tereschenko, VP, Lopatnikova, JA, Obleukhova, IA & Sennikov, SV 2019, 'The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice', Journal of Immunology Research, Том. 2019, 7029726, стр. 7029726. https://doi.org/10.1155/2019/7029726

APA

Kurilin, V. V., Khantakova, J. N., Tereschenko, V. P., Lopatnikova, J. A., Obleukhova, I. A., & Sennikov, S. V. (2019). The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice. Journal of Immunology Research, 2019, 7029726. [7029726]. https://doi.org/10.1155/2019/7029726

Vancouver

Kurilin VV, Khantakova JN, Tereschenko VP, Lopatnikova JA, Obleukhova IA, Sennikov SV. The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice. Journal of Immunology Research. 2019 янв. 1;2019:7029726. 7029726. doi: 10.1155/2019/7029726

Author

Kurilin, Vasiliy V. ; Khantakova, Julia N. ; Tereschenko, Valeriy P. и др. / The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice. в: Journal of Immunology Research. 2019 ; Том 2019. стр. 7029726.

BibTeX

@article{76e0836bfb254846a98d7e5363e71f09,
title = "The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice",
abstract = "Introduction: Dendritic cells (DCs) control immune responses by modulating T and B cells towards effector or tolerogenic responses. In this study, we evaluated the effects of different immunosuppressive molecules on the phenotypic and functional characteristics of primary dendritic cells from C57BL/6 and CBA mice. Methods: DCs were derived from bone marrow cells in the presence of rmGM-CSF and rmIL-4. DCs were then treated with different types of immunosuppressive molecules (rmIL-10, rmTGF-β, and BAY 11-7082) and cocultured with syngeneic splenocytes. The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were evaluated. Results: Tolerogenic factors were found to have different effects on DCs C57Bl/6 mice. In C57Bl/6 mice, BAY 11-7082 alone had no effect on the expression of DC maturation molecules (CD80, CD86). Transforming growth factor beta (TGF-β), alone and in combination with BAY 11-7082, reduced the expression of these molecules. Cocultivation of DCs with splenocytes in the presence of TGF-β and BAY 11-7082 favored regulatory T cell (CD4+CD25hiFoxP3+) differentiation and disfavored differentiation of CD4+ T cells producing IL-10. In CBA mice, we found that rmIL-10 and rmTGF-β have a weak effect on maturation of DCs and their functional properties to induce Treg cells and IL-10 production. Conclusion: These results indicate that TGF-β and IL-10 have different effects on the phenotypic and functional characteristics of DCs and that the NF-κB inhibitor, BAY 11-7082, has no synergistic effect on these treatments. In mice with an opposite nature of the immune response, the effects of immunoregulatory cytokines (IL-10 and TGF-b) differ on maturation of dendritic cells.",
keywords = "INDUCTION, TOLERANCE",
author = "Kurilin, {Vasiliy V.} and Khantakova, {Julia N.} and Tereschenko, {Valeriy P.} and Lopatnikova, {Julia A.} and Obleukhova, {Irina A.} and Sennikov, {Sergey V.}",
note = "Publisher Copyright: {\textcopyright} 2019 Vasiliy V. Kurilin et al.",
year = "2019",
month = jan,
day = "1",
doi = "10.1155/2019/7029726",
language = "English",
volume = "2019",
pages = "7029726",
journal = "Journal of Immunology Research",
issn = "2314-8861",
publisher = "Hindawi Limited",

}

RIS

TY - JOUR

T1 - The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice

AU - Kurilin, Vasiliy V.

AU - Khantakova, Julia N.

AU - Tereschenko, Valeriy P.

AU - Lopatnikova, Julia A.

AU - Obleukhova, Irina A.

AU - Sennikov, Sergey V.

N1 - Publisher Copyright: © 2019 Vasiliy V. Kurilin et al.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Introduction: Dendritic cells (DCs) control immune responses by modulating T and B cells towards effector or tolerogenic responses. In this study, we evaluated the effects of different immunosuppressive molecules on the phenotypic and functional characteristics of primary dendritic cells from C57BL/6 and CBA mice. Methods: DCs were derived from bone marrow cells in the presence of rmGM-CSF and rmIL-4. DCs were then treated with different types of immunosuppressive molecules (rmIL-10, rmTGF-β, and BAY 11-7082) and cocultured with syngeneic splenocytes. The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were evaluated. Results: Tolerogenic factors were found to have different effects on DCs C57Bl/6 mice. In C57Bl/6 mice, BAY 11-7082 alone had no effect on the expression of DC maturation molecules (CD80, CD86). Transforming growth factor beta (TGF-β), alone and in combination with BAY 11-7082, reduced the expression of these molecules. Cocultivation of DCs with splenocytes in the presence of TGF-β and BAY 11-7082 favored regulatory T cell (CD4+CD25hiFoxP3+) differentiation and disfavored differentiation of CD4+ T cells producing IL-10. In CBA mice, we found that rmIL-10 and rmTGF-β have a weak effect on maturation of DCs and their functional properties to induce Treg cells and IL-10 production. Conclusion: These results indicate that TGF-β and IL-10 have different effects on the phenotypic and functional characteristics of DCs and that the NF-κB inhibitor, BAY 11-7082, has no synergistic effect on these treatments. In mice with an opposite nature of the immune response, the effects of immunoregulatory cytokines (IL-10 and TGF-b) differ on maturation of dendritic cells.

AB - Introduction: Dendritic cells (DCs) control immune responses by modulating T and B cells towards effector or tolerogenic responses. In this study, we evaluated the effects of different immunosuppressive molecules on the phenotypic and functional characteristics of primary dendritic cells from C57BL/6 and CBA mice. Methods: DCs were derived from bone marrow cells in the presence of rmGM-CSF and rmIL-4. DCs were then treated with different types of immunosuppressive molecules (rmIL-10, rmTGF-β, and BAY 11-7082) and cocultured with syngeneic splenocytes. The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were evaluated. Results: Tolerogenic factors were found to have different effects on DCs C57Bl/6 mice. In C57Bl/6 mice, BAY 11-7082 alone had no effect on the expression of DC maturation molecules (CD80, CD86). Transforming growth factor beta (TGF-β), alone and in combination with BAY 11-7082, reduced the expression of these molecules. Cocultivation of DCs with splenocytes in the presence of TGF-β and BAY 11-7082 favored regulatory T cell (CD4+CD25hiFoxP3+) differentiation and disfavored differentiation of CD4+ T cells producing IL-10. In CBA mice, we found that rmIL-10 and rmTGF-β have a weak effect on maturation of DCs and their functional properties to induce Treg cells and IL-10 production. Conclusion: These results indicate that TGF-β and IL-10 have different effects on the phenotypic and functional characteristics of DCs and that the NF-κB inhibitor, BAY 11-7082, has no synergistic effect on these treatments. In mice with an opposite nature of the immune response, the effects of immunoregulatory cytokines (IL-10 and TGF-b) differ on maturation of dendritic cells.

KW - INDUCTION

KW - TOLERANCE

UR - http://www.scopus.com/inward/record.url?scp=85067276430&partnerID=8YFLogxK

U2 - 10.1155/2019/7029726

DO - 10.1155/2019/7029726

M3 - Article

C2 - 31143783

AN - SCOPUS:85067276430

VL - 2019

SP - 7029726

JO - Journal of Immunology Research

JF - Journal of Immunology Research

SN - 2314-8861

M1 - 7029726

ER -

ID: 20591529