Research output: Contribution to journal › Article › peer-review
The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice. / Kurilin, Vasiliy V.; Khantakova, Julia N.; Tereschenko, Valeriy P. et al.
In: Journal of Immunology Research, Vol. 2019, 7029726, 01.01.2019, p. 7029726.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice
AU - Kurilin, Vasiliy V.
AU - Khantakova, Julia N.
AU - Tereschenko, Valeriy P.
AU - Lopatnikova, Julia A.
AU - Obleukhova, Irina A.
AU - Sennikov, Sergey V.
N1 - Publisher Copyright: © 2019 Vasiliy V. Kurilin et al.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Introduction: Dendritic cells (DCs) control immune responses by modulating T and B cells towards effector or tolerogenic responses. In this study, we evaluated the effects of different immunosuppressive molecules on the phenotypic and functional characteristics of primary dendritic cells from C57BL/6 and CBA mice. Methods: DCs were derived from bone marrow cells in the presence of rmGM-CSF and rmIL-4. DCs were then treated with different types of immunosuppressive molecules (rmIL-10, rmTGF-β, and BAY 11-7082) and cocultured with syngeneic splenocytes. The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were evaluated. Results: Tolerogenic factors were found to have different effects on DCs C57Bl/6 mice. In C57Bl/6 mice, BAY 11-7082 alone had no effect on the expression of DC maturation molecules (CD80, CD86). Transforming growth factor beta (TGF-β), alone and in combination with BAY 11-7082, reduced the expression of these molecules. Cocultivation of DCs with splenocytes in the presence of TGF-β and BAY 11-7082 favored regulatory T cell (CD4+CD25hiFoxP3+) differentiation and disfavored differentiation of CD4+ T cells producing IL-10. In CBA mice, we found that rmIL-10 and rmTGF-β have a weak effect on maturation of DCs and their functional properties to induce Treg cells and IL-10 production. Conclusion: These results indicate that TGF-β and IL-10 have different effects on the phenotypic and functional characteristics of DCs and that the NF-κB inhibitor, BAY 11-7082, has no synergistic effect on these treatments. In mice with an opposite nature of the immune response, the effects of immunoregulatory cytokines (IL-10 and TGF-b) differ on maturation of dendritic cells.
AB - Introduction: Dendritic cells (DCs) control immune responses by modulating T and B cells towards effector or tolerogenic responses. In this study, we evaluated the effects of different immunosuppressive molecules on the phenotypic and functional characteristics of primary dendritic cells from C57BL/6 and CBA mice. Methods: DCs were derived from bone marrow cells in the presence of rmGM-CSF and rmIL-4. DCs were then treated with different types of immunosuppressive molecules (rmIL-10, rmTGF-β, and BAY 11-7082) and cocultured with syngeneic splenocytes. The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were evaluated. Results: Tolerogenic factors were found to have different effects on DCs C57Bl/6 mice. In C57Bl/6 mice, BAY 11-7082 alone had no effect on the expression of DC maturation molecules (CD80, CD86). Transforming growth factor beta (TGF-β), alone and in combination with BAY 11-7082, reduced the expression of these molecules. Cocultivation of DCs with splenocytes in the presence of TGF-β and BAY 11-7082 favored regulatory T cell (CD4+CD25hiFoxP3+) differentiation and disfavored differentiation of CD4+ T cells producing IL-10. In CBA mice, we found that rmIL-10 and rmTGF-β have a weak effect on maturation of DCs and their functional properties to induce Treg cells and IL-10 production. Conclusion: These results indicate that TGF-β and IL-10 have different effects on the phenotypic and functional characteristics of DCs and that the NF-κB inhibitor, BAY 11-7082, has no synergistic effect on these treatments. In mice with an opposite nature of the immune response, the effects of immunoregulatory cytokines (IL-10 and TGF-b) differ on maturation of dendritic cells.
KW - INDUCTION
KW - TOLERANCE
UR - http://www.scopus.com/inward/record.url?scp=85067276430&partnerID=8YFLogxK
U2 - 10.1155/2019/7029726
DO - 10.1155/2019/7029726
M3 - Article
C2 - 31143783
AN - SCOPUS:85067276430
VL - 2019
SP - 7029726
JO - Journal of Immunology Research
JF - Journal of Immunology Research
SN - 2314-8861
M1 - 7029726
ER -
ID: 20591529