TY - JOUR

T1 - The effect of blood contained in the samples on the metabolomic profile of mouse brain tissue

T2 - A study by NMR spectroscopy

AU - Glinskikh, Anastasia

AU - Snytnikova, Olga

AU - Zelentsova, Ekaterina

AU - Borisova, Maria

AU - Tsentalovich, Yuri

AU - Akulov, Andrey

N1 - Funding Information: Acknowledgments: We thank the Ministry of Science and Higher Education of the RF for the access to NMR equipment and the Center for Genetic Resources of Laboratory Animals at ICG SB RAS (Unique identifier of the project RFMEFI62119X0023). Funding Information: The study was carried out at the expense of a grant from the Russian Foundation for Basic Research (project No. 19-315-90008). The maintenance of the genetic line of CD-1 mice was carried out at the expense of state order No. 0259-2019-0004-C-01. OS and YT thank Russian Foundation for Basic Research (Project 20-03-00234) for NMR measurements. Funding Information: Funding: The study was carried out at the expense of a grant from the Russian Foundation for Basic Research (project No. 19-315-90008). The maintenance of the genetic line of CD-1 mice was carried out at the expense of state order No. 0259-2019-0004-C-01. OS and YT thank Russian Foundation for Basic Research (Project 20-03-00234) for NMR measurements. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/6/1

Y1 - 2021/6/1

N2 - (1) Recently, metabolic profiling of the tissue in the native state or extracts of its metabolites has become increasingly important in the field of metabolomics. An important factor, in this case, is the presence of blood in a tissue sample, which can potentially lead to a change in the concentration of tissue metabolites and, as a result, distortion of experimental data and their interpretation. (2) In this paper, the metabolomic profiling based on NMR spectroscopy was performed to determine the effect of blood contained in the studied samples of brain tissue on their metabolomic profile. We used 13 male laboratory CD-1® IGS mice for this study. The animals were divided into two groups. The first group of animals (n = 7) was subjected to the perfusion procedure, and the second group of animals (n = 6) was not perfused. The brain tissues of the animals were homogenized, and the metabolite fraction was extracted with a water/methanol/chloroform solution. Samples were studied by high-frequency 1H-NMR spectroscopy with subsequent statistical data analysis. The group comparison was performed with the use of the Student’s test. We identified 36 metabolites in the brain tissue with the use of NMR spectroscopy. (3) For the major set of studied metabolites, no significant differences were found in the brain tissue metabolite concentrations in the native state and after the blood removal procedure. (4) Thus, it was shown that the presence of blood does not have a significant effect on the metabolomic profile of the brain in animals without pathologies.

AB - (1) Recently, metabolic profiling of the tissue in the native state or extracts of its metabolites has become increasingly important in the field of metabolomics. An important factor, in this case, is the presence of blood in a tissue sample, which can potentially lead to a change in the concentration of tissue metabolites and, as a result, distortion of experimental data and their interpretation. (2) In this paper, the metabolomic profiling based on NMR spectroscopy was performed to determine the effect of blood contained in the studied samples of brain tissue on their metabolomic profile. We used 13 male laboratory CD-1® IGS mice for this study. The animals were divided into two groups. The first group of animals (n = 7) was subjected to the perfusion procedure, and the second group of animals (n = 6) was not perfused. The brain tissues of the animals were homogenized, and the metabolite fraction was extracted with a water/methanol/chloroform solution. Samples were studied by high-frequency 1H-NMR spectroscopy with subsequent statistical data analysis. The group comparison was performed with the use of the Student’s test. We identified 36 metabolites in the brain tissue with the use of NMR spectroscopy. (3) For the major set of studied metabolites, no significant differences were found in the brain tissue metabolite concentrations in the native state and after the blood removal procedure. (4) Thus, it was shown that the presence of blood does not have a significant effect on the metabolomic profile of the brain in animals without pathologies.

KW - Brain tissue

KW - Metabolomic profile

KW - Metabolomics

KW - Nuclear magnetic resonance

KW - Magnetic Resonance Spectroscopy

KW - Metabolome

KW - Male

KW - Water/chemistry

KW - Blood

KW - Brain/drug effects

KW - Specimen Handling

KW - Methanol/chemistry

KW - Animals

KW - Biocompatible Materials

KW - Chloroform/chemistry

KW - Mice

UR - http://www.scopus.com/inward/record.url?scp=85107129882&partnerID=8YFLogxK

U2 - 10.3390/molecules26113096

DO - 10.3390/molecules26113096

M3 - Article

C2 - 34067246

AN - SCOPUS:85107129882

VL - 26

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 11

M1 - 3096

ER -