Research output: Contribution to journal › Article › peer-review
The effect of blood contained in the samples on the metabolomic profile of mouse brain tissue : A study by NMR spectroscopy. / Glinskikh, Anastasia; Snytnikova, Olga; Zelentsova, Ekaterina et al.
In: Molecules, Vol. 26, No. 11, 3096, 01.06.2021.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The effect of blood contained in the samples on the metabolomic profile of mouse brain tissue
T2 - A study by NMR spectroscopy
AU - Glinskikh, Anastasia
AU - Snytnikova, Olga
AU - Zelentsova, Ekaterina
AU - Borisova, Maria
AU - Tsentalovich, Yuri
AU - Akulov, Andrey
N1 - Funding Information: Acknowledgments: We thank the Ministry of Science and Higher Education of the RF for the access to NMR equipment and the Center for Genetic Resources of Laboratory Animals at ICG SB RAS (Unique identifier of the project RFMEFI62119X0023). Funding Information: The study was carried out at the expense of a grant from the Russian Foundation for Basic Research (project No. 19-315-90008). The maintenance of the genetic line of CD-1 mice was carried out at the expense of state order No. 0259-2019-0004-C-01. OS and YT thank Russian Foundation for Basic Research (Project 20-03-00234) for NMR measurements. Funding Information: Funding: The study was carried out at the expense of a grant from the Russian Foundation for Basic Research (project No. 19-315-90008). The maintenance of the genetic line of CD-1 mice was carried out at the expense of state order No. 0259-2019-0004-C-01. OS and YT thank Russian Foundation for Basic Research (Project 20-03-00234) for NMR measurements. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/6/1
Y1 - 2021/6/1
N2 - (1) Recently, metabolic profiling of the tissue in the native state or extracts of its metabolites has become increasingly important in the field of metabolomics. An important factor, in this case, is the presence of blood in a tissue sample, which can potentially lead to a change in the concentration of tissue metabolites and, as a result, distortion of experimental data and their interpretation. (2) In this paper, the metabolomic profiling based on NMR spectroscopy was performed to determine the effect of blood contained in the studied samples of brain tissue on their metabolomic profile. We used 13 male laboratory CD-1® IGS mice for this study. The animals were divided into two groups. The first group of animals (n = 7) was subjected to the perfusion procedure, and the second group of animals (n = 6) was not perfused. The brain tissues of the animals were homogenized, and the metabolite fraction was extracted with a water/methanol/chloroform solution. Samples were studied by high-frequency 1H-NMR spectroscopy with subsequent statistical data analysis. The group comparison was performed with the use of the Student’s test. We identified 36 metabolites in the brain tissue with the use of NMR spectroscopy. (3) For the major set of studied metabolites, no significant differences were found in the brain tissue metabolite concentrations in the native state and after the blood removal procedure. (4) Thus, it was shown that the presence of blood does not have a significant effect on the metabolomic profile of the brain in animals without pathologies.
AB - (1) Recently, metabolic profiling of the tissue in the native state or extracts of its metabolites has become increasingly important in the field of metabolomics. An important factor, in this case, is the presence of blood in a tissue sample, which can potentially lead to a change in the concentration of tissue metabolites and, as a result, distortion of experimental data and their interpretation. (2) In this paper, the metabolomic profiling based on NMR spectroscopy was performed to determine the effect of blood contained in the studied samples of brain tissue on their metabolomic profile. We used 13 male laboratory CD-1® IGS mice for this study. The animals were divided into two groups. The first group of animals (n = 7) was subjected to the perfusion procedure, and the second group of animals (n = 6) was not perfused. The brain tissues of the animals were homogenized, and the metabolite fraction was extracted with a water/methanol/chloroform solution. Samples were studied by high-frequency 1H-NMR spectroscopy with subsequent statistical data analysis. The group comparison was performed with the use of the Student’s test. We identified 36 metabolites in the brain tissue with the use of NMR spectroscopy. (3) For the major set of studied metabolites, no significant differences were found in the brain tissue metabolite concentrations in the native state and after the blood removal procedure. (4) Thus, it was shown that the presence of blood does not have a significant effect on the metabolomic profile of the brain in animals without pathologies.
KW - Brain tissue
KW - Metabolomic profile
KW - Metabolomics
KW - Nuclear magnetic resonance
KW - Magnetic Resonance Spectroscopy
KW - Metabolome
KW - Male
KW - Water/chemistry
KW - Blood
KW - Brain/drug effects
KW - Specimen Handling
KW - Methanol/chemistry
KW - Animals
KW - Biocompatible Materials
KW - Chloroform/chemistry
KW - Mice
UR - http://www.scopus.com/inward/record.url?scp=85107129882&partnerID=8YFLogxK
U2 - 10.3390/molecules26113096
DO - 10.3390/molecules26113096
M3 - Article
C2 - 34067246
AN - SCOPUS:85107129882
VL - 26
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 11
M1 - 3096
ER -
ID: 29238768