Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
The effect of 16S rRNA region choice on bacterial community metabarcoding results. / Букин, Юрий; Галачьянц, Юрий; Морозов, Игорь Владимирович и др.
в: Scientific Data, Том 6, 190007, 05.02.2019, стр. 190007.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - The effect of 16S rRNA region choice on bacterial community metabarcoding results
AU - Букин, Юрий
AU - Галачьянц, Юрий
AU - Морозов, Игорь Владимирович
AU - Букин, Сергей
AU - Захаренко, А.
AU - Земская, Тамара
N1 - Publisher Copyright: © The Author(s) 2019. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/2/5
Y1 - 2019/2/5
N2 - In this work, we compare the resolution of V2-V3 and V3-V4 16S rRNA regions for the purposes of estimating microbial community diversity using paired-end Illumina MiSeq reads, and show that the fragment, including V2 and V3 regions, has higher resolution for lower-rank taxa (genera and species). It allows for a more precise distance-based clustering of reads into species-level OTUs. Statistically convergent estimates of the diversity of major species (defined as those that together are covered by 95% of reads) can be achieved at the sample sizes of 10000 to 15000 reads. The relative error of the Shannon index estimate for this condition is lower than 4%.
AB - In this work, we compare the resolution of V2-V3 and V3-V4 16S rRNA regions for the purposes of estimating microbial community diversity using paired-end Illumina MiSeq reads, and show that the fragment, including V2 and V3 regions, has higher resolution for lower-rank taxa (genera and species). It allows for a more precise distance-based clustering of reads into species-level OTUs. Statistically convergent estimates of the diversity of major species (defined as those that together are covered by 95% of reads) can be achieved at the sample sizes of 10000 to 15000 reads. The relative error of the Shannon index estimate for this condition is lower than 4%.
KW - Bacteria/genetics
KW - Computational Biology/methods
KW - DNA Barcoding, Taxonomic/methods
KW - High-Throughput Nucleotide Sequencing
KW - Lakes
KW - Metagenomics/methods
KW - Microbiota/genetics
KW - RNA, Ribosomal, 16S/genetics
KW - Russia
KW - Sequence Analysis, DNA
KW - Water Microbiology
UR - http://www.scopus.com/inward/record.url?scp=85061060066&partnerID=8YFLogxK
U2 - 10.1038/sdata.2019.7
DO - 10.1038/sdata.2019.7
M3 - Article
C2 - 30720800
VL - 6
SP - 190007
JO - Scientific Data
JF - Scientific Data
SN - 2052-4463
M1 - 190007
ER -
ID: 25311878