Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Technology of regeneration of tje bone tissue in a rotational bioreactor : fluid flow simulation and laser-induced fluorescence diagnostics. / Tsibulskaya, E. O.; Maslov, N. A.; Larionov, P. M. и др.
в: Journal of Applied Mechanics and Technical Physics, Том 61, № 5, 09.2020, стр. 776-786.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Technology of regeneration of tje bone tissue in a rotational bioreactor
T2 - fluid flow simulation and laser-induced fluorescence diagnostics
AU - Tsibulskaya, E. O.
AU - Maslov, N. A.
AU - Larionov, P. M.
AU - Ganimedov, V. L.
N1 - Funding Information: This work was performed within the framework of the Program of Fundamental Scientific Research of the State Academies of Sciences in 2013–2020 (Grant Nos. AAAA-A17-117030610126-4 and AAAA-A17-117030610122-6). Publisher Copyright: © 2020, Pleiades Publishing, Ltd. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/9
Y1 - 2020/9
N2 - A technology of bone tissue cultivation on a thin scaffold in a rotational bioreactor is developed. An optimal regime of cell material cultivation is determined for testing the method, and an optical method for diagnostics of the bone growth dynamics during its cultivation is found. Numerical simulations of the fluid flow in the bioreactor make it possible to significantly simplify the medical experiment and to choose the optimal values of the rotation frequencies and shear stresses acting on the cell material placed on the scaffold. The optical diagnostics of the scaffold samples in the course of dynamic cultivation in the bioreactor is performed by the method of laser-induced fluorescence. An algorithm based on the principal component analysis is applied to analyze the spectral data; as a result, the spectra of excitation and fluorescent emission of the basic fluorescent substances in the sample (tyrosine and tryptophan amino acids, structural protein (collagen), and fluorescent structures of polycaprolactone) are calculated. It is found that the contribution of the component corresponding to collagen in the samples increases in the process of dynamic cultivation, which testifies to effective formation of the extracellular matrix of the bone.
AB - A technology of bone tissue cultivation on a thin scaffold in a rotational bioreactor is developed. An optimal regime of cell material cultivation is determined for testing the method, and an optical method for diagnostics of the bone growth dynamics during its cultivation is found. Numerical simulations of the fluid flow in the bioreactor make it possible to significantly simplify the medical experiment and to choose the optimal values of the rotation frequencies and shear stresses acting on the cell material placed on the scaffold. The optical diagnostics of the scaffold samples in the course of dynamic cultivation in the bioreactor is performed by the method of laser-induced fluorescence. An algorithm based on the principal component analysis is applied to analyze the spectral data; as a result, the spectra of excitation and fluorescent emission of the basic fluorescent substances in the sample (tyrosine and tryptophan amino acids, structural protein (collagen), and fluorescent structures of polycaprolactone) are calculated. It is found that the contribution of the component corresponding to collagen in the samples increases in the process of dynamic cultivation, which testifies to effective formation of the extracellular matrix of the bone.
KW - computational algorithm
KW - laminar flow regime
KW - laser-induced fluorescence
KW - mathematical modeling
KW - Navier–Stokes equations
KW - principal component analysis
KW - rotational bioreactor
KW - scaffold
KW - shear stress
KW - Taylor vortices
KW - CULTURE
KW - Stokes equations
KW - Navier–
UR - http://www.scopus.com/inward/record.url?scp=85097061610&partnerID=8YFLogxK
U2 - 10.1134/S0021894420050120
DO - 10.1134/S0021894420050120
M3 - Article
AN - SCOPUS:85097061610
VL - 61
SP - 776
EP - 786
JO - Journal of Applied Mechanics and Technical Physics
JF - Journal of Applied Mechanics and Technical Physics
SN - 0021-8944
IS - 5
ER -
ID: 26203314