TY - JOUR

T1 - Technology of regeneration of tje bone tissue in a rotational bioreactor

T2 - fluid flow simulation and laser-induced fluorescence diagnostics

AU - Tsibulskaya, E. O.

AU - Maslov, N. A.

AU - Larionov, P. M.

AU - Ganimedov, V. L.

N1 - Funding Information: This work was performed within the framework of the Program of Fundamental Scientific Research of the State Academies of Sciences in 2013–2020 (Grant Nos. AAAA-A17-117030610126-4 and AAAA-A17-117030610122-6). Publisher Copyright: © 2020, Pleiades Publishing, Ltd. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/9

Y1 - 2020/9

N2 - A technology of bone tissue cultivation on a thin scaffold in a rotational bioreactor is developed. An optimal regime of cell material cultivation is determined for testing the method, and an optical method for diagnostics of the bone growth dynamics during its cultivation is found. Numerical simulations of the fluid flow in the bioreactor make it possible to significantly simplify the medical experiment and to choose the optimal values of the rotation frequencies and shear stresses acting on the cell material placed on the scaffold. The optical diagnostics of the scaffold samples in the course of dynamic cultivation in the bioreactor is performed by the method of laser-induced fluorescence. An algorithm based on the principal component analysis is applied to analyze the spectral data; as a result, the spectra of excitation and fluorescent emission of the basic fluorescent substances in the sample (tyrosine and tryptophan amino acids, structural protein (collagen), and fluorescent structures of polycaprolactone) are calculated. It is found that the contribution of the component corresponding to collagen in the samples increases in the process of dynamic cultivation, which testifies to effective formation of the extracellular matrix of the bone.

AB - A technology of bone tissue cultivation on a thin scaffold in a rotational bioreactor is developed. An optimal regime of cell material cultivation is determined for testing the method, and an optical method for diagnostics of the bone growth dynamics during its cultivation is found. Numerical simulations of the fluid flow in the bioreactor make it possible to significantly simplify the medical experiment and to choose the optimal values of the rotation frequencies and shear stresses acting on the cell material placed on the scaffold. The optical diagnostics of the scaffold samples in the course of dynamic cultivation in the bioreactor is performed by the method of laser-induced fluorescence. An algorithm based on the principal component analysis is applied to analyze the spectral data; as a result, the spectra of excitation and fluorescent emission of the basic fluorescent substances in the sample (tyrosine and tryptophan amino acids, structural protein (collagen), and fluorescent structures of polycaprolactone) are calculated. It is found that the contribution of the component corresponding to collagen in the samples increases in the process of dynamic cultivation, which testifies to effective formation of the extracellular matrix of the bone.

KW - computational algorithm

KW - laminar flow regime

KW - laser-induced fluorescence

KW - mathematical modeling

KW - Navier–Stokes equations

KW - principal component analysis

KW - rotational bioreactor

KW - scaffold

KW - shear stress

KW - Taylor vortices

KW - CULTURE

KW - Stokes equations

KW - Navier&#8211

UR - http://www.scopus.com/inward/record.url?scp=85097061610&partnerID=8YFLogxK

U2 - 10.1134/S0021894420050120

DO - 10.1134/S0021894420050120

M3 - Article

AN - SCOPUS:85097061610

VL - 61

SP - 776

EP - 786

JO - Journal of Applied Mechanics and Technical Physics

JF - Journal of Applied Mechanics and Technical Physics

SN - 0021-8944

IS - 5

ER -