TY - JOUR

T1 - StM171, a Stenotrophomonas maltophilia Bacteriophage That Affects Sensitivity to Antibiotics in Host Bacteria and Their Biofilm Formation

AU - Jdeed, Ghadeer

AU - Morozova, Vera

AU - Kozlova, Yuliya

AU - Tikunov, Artem

AU - Ushakova, Tatyana

AU - Bardasheva, Alevtina

AU - Manakhov, Andrey

AU - Mitina, Maria

AU - Zhirakovskaya, Elena

AU - Tikunova, Nina

N1 - This study was supported by the Ministry of Science and Higher Education of the Russian Federation under project grant No. 075-15-2021-1085.

PY - 2023/12

Y1 - 2023/12

N2 - Stenotrophomonas maltophilia mainly causes respiratory infections that are associated with a high mortality rate among immunocompromised patients. S. maltophilia exhibits a high level of antibiotic resistance and can form biofilms, which complicates the treatment of patients infected with this bacterium. Phages combined with antibiotics could be a promising treatment option. Currently, ~60 S. maltophilia phages are known, and their effects on biofilm formation and antibiotic sensitivity require further examination. Bacteriophage StM171, which was isolated from hospital wastewater, showed a medium host range, low burst size, and low lytic activity. StM171 has a 44kbp dsDNA genome that encodes 59 open-reading frames. A comparative genomic analysis indicated that StM171, along with the Stenotrophomonas phage Suso (MZ326866) and Xanthomonas phage HXX_Dennis (ON711490), are members of a new putative Nordvirus genus. S. maltophilia strains that developed resistance to StM171 (bacterial-insensitive mutants) showed a changed sensitivity to antibiotics compared to the originally susceptible strains. Some bacterial-insensitive mutants restored sensitivity to cephalosporin and penicillin-like antibiotics and became resistant to erythromycin. StM171 shows strain- and antibiotic-dependent effects on the biofilm formation of S. maltophilia strains.

AB - Stenotrophomonas maltophilia mainly causes respiratory infections that are associated with a high mortality rate among immunocompromised patients. S. maltophilia exhibits a high level of antibiotic resistance and can form biofilms, which complicates the treatment of patients infected with this bacterium. Phages combined with antibiotics could be a promising treatment option. Currently, ~60 S. maltophilia phages are known, and their effects on biofilm formation and antibiotic sensitivity require further examination. Bacteriophage StM171, which was isolated from hospital wastewater, showed a medium host range, low burst size, and low lytic activity. StM171 has a 44kbp dsDNA genome that encodes 59 open-reading frames. A comparative genomic analysis indicated that StM171, along with the Stenotrophomonas phage Suso (MZ326866) and Xanthomonas phage HXX_Dennis (ON711490), are members of a new putative Nordvirus genus. S. maltophilia strains that developed resistance to StM171 (bacterial-insensitive mutants) showed a changed sensitivity to antibiotics compared to the originally susceptible strains. Some bacterial-insensitive mutants restored sensitivity to cephalosporin and penicillin-like antibiotics and became resistant to erythromycin. StM171 shows strain- and antibiotic-dependent effects on the biofilm formation of S. maltophilia strains.

KW - Humans

KW - Anti-Bacterial Agents/pharmacology

KW - Bacteriophages/genetics

KW - Stenotrophomonas maltophilia/genetics

KW - Biofilms

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85180491880&origin=inward&txGid=d243657524a5678696284e46eb77290e

U2 - 10.3390/v15122455

DO - 10.3390/v15122455

M3 - Article

C2 - 38140696

VL - 15

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 12

M1 - 2455

ER -