Research output: Contribution to journal › Article › peer-review
StM171, a Stenotrophomonas maltophilia Bacteriophage That Affects Sensitivity to Antibiotics in Host Bacteria and Their Biofilm Formation. / Jdeed, Ghadeer; Morozova, Vera; Kozlova, Yuliya et al.
In: Viruses, Vol. 15, No. 12, 2455, 12.2023.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - StM171, a Stenotrophomonas maltophilia Bacteriophage That Affects Sensitivity to Antibiotics in Host Bacteria and Their Biofilm Formation
AU - Jdeed, Ghadeer
AU - Morozova, Vera
AU - Kozlova, Yuliya
AU - Tikunov, Artem
AU - Ushakova, Tatyana
AU - Bardasheva, Alevtina
AU - Manakhov, Andrey
AU - Mitina, Maria
AU - Zhirakovskaya, Elena
AU - Tikunova, Nina
N1 - This study was supported by the Ministry of Science and Higher Education of the Russian Federation under project grant No. 075-15-2021-1085.
PY - 2023/12
Y1 - 2023/12
N2 - Stenotrophomonas maltophilia mainly causes respiratory infections that are associated with a high mortality rate among immunocompromised patients. S. maltophilia exhibits a high level of antibiotic resistance and can form biofilms, which complicates the treatment of patients infected with this bacterium. Phages combined with antibiotics could be a promising treatment option. Currently, ~60 S. maltophilia phages are known, and their effects on biofilm formation and antibiotic sensitivity require further examination. Bacteriophage StM171, which was isolated from hospital wastewater, showed a medium host range, low burst size, and low lytic activity. StM171 has a 44kbp dsDNA genome that encodes 59 open-reading frames. A comparative genomic analysis indicated that StM171, along with the Stenotrophomonas phage Suso (MZ326866) and Xanthomonas phage HXX_Dennis (ON711490), are members of a new putative Nordvirus genus. S. maltophilia strains that developed resistance to StM171 (bacterial-insensitive mutants) showed a changed sensitivity to antibiotics compared to the originally susceptible strains. Some bacterial-insensitive mutants restored sensitivity to cephalosporin and penicillin-like antibiotics and became resistant to erythromycin. StM171 shows strain- and antibiotic-dependent effects on the biofilm formation of S. maltophilia strains.
AB - Stenotrophomonas maltophilia mainly causes respiratory infections that are associated with a high mortality rate among immunocompromised patients. S. maltophilia exhibits a high level of antibiotic resistance and can form biofilms, which complicates the treatment of patients infected with this bacterium. Phages combined with antibiotics could be a promising treatment option. Currently, ~60 S. maltophilia phages are known, and their effects on biofilm formation and antibiotic sensitivity require further examination. Bacteriophage StM171, which was isolated from hospital wastewater, showed a medium host range, low burst size, and low lytic activity. StM171 has a 44kbp dsDNA genome that encodes 59 open-reading frames. A comparative genomic analysis indicated that StM171, along with the Stenotrophomonas phage Suso (MZ326866) and Xanthomonas phage HXX_Dennis (ON711490), are members of a new putative Nordvirus genus. S. maltophilia strains that developed resistance to StM171 (bacterial-insensitive mutants) showed a changed sensitivity to antibiotics compared to the originally susceptible strains. Some bacterial-insensitive mutants restored sensitivity to cephalosporin and penicillin-like antibiotics and became resistant to erythromycin. StM171 shows strain- and antibiotic-dependent effects on the biofilm formation of S. maltophilia strains.
KW - Humans
KW - Anti-Bacterial Agents/pharmacology
KW - Bacteriophages/genetics
KW - Stenotrophomonas maltophilia/genetics
KW - Biofilms
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85180491880&origin=inward&txGid=d243657524a5678696284e46eb77290e
U2 - 10.3390/v15122455
DO - 10.3390/v15122455
M3 - Article
C2 - 38140696
VL - 15
JO - Viruses
JF - Viruses
SN - 1999-4915
IS - 12
M1 - 2455
ER -
ID: 59534988