TY - JOUR

T1 - Sterile Fecal Microbiota Transplantation Boosts Anti-Inflammatory T-Cell Response in Ulcerative Colitis Patients

AU - Chechushkov, Anton

AU - Desyukevich, Pavel

AU - Yakovlev, Timir

AU - Al Allaf, Lina

AU - Shrainer, Evgeniya

AU - Morozov, Vitalyi

AU - Tikunova, Nina

N1 - All immunological investigations were funded by the Russian Science Foundation, Project No. 21-14-00360. Blood and stool sampling was supported by the Ministry of Education and Science, Project No. 121031300043-8.

PY - 2024/2

Y1 - 2024/2

N2 - Ulcerative colitis is a chronic immune-mediated disease of unclear etiology, affecting people of different ages and significantly reducing the quality of life. Modern methods of therapy are mainly represented by anti-inflammatory drugs and are not aimed at a specific pathogenetic factor. In this study, we investigated the effect of transplantation of sterile stool filtrate from healthy donors on the induction of anti-inflammatory immune mechanisms. It was shown that performing such a procedure in patients with ulcerative colitis caused the appearance of T helper cells in the blood, which reacted to the content of sterile stool filtrates in an antigen-specific manner and produced IL-10. At the same time, cells of the same patients before therapy in response to the addition of sterile stool filtrates were less reactive and predominantly produced IL-4, indicating its pro-inflammatory skewing. The obtained data demonstrated the effect of an anti-inflammatory shift in the T-helper response after transplantation of sterile stool filtrate, which increased and persisted for at least three months after the procedure.

AB - Ulcerative colitis is a chronic immune-mediated disease of unclear etiology, affecting people of different ages and significantly reducing the quality of life. Modern methods of therapy are mainly represented by anti-inflammatory drugs and are not aimed at a specific pathogenetic factor. In this study, we investigated the effect of transplantation of sterile stool filtrate from healthy donors on the induction of anti-inflammatory immune mechanisms. It was shown that performing such a procedure in patients with ulcerative colitis caused the appearance of T helper cells in the blood, which reacted to the content of sterile stool filtrates in an antigen-specific manner and produced IL-10. At the same time, cells of the same patients before therapy in response to the addition of sterile stool filtrates were less reactive and predominantly produced IL-4, indicating its pro-inflammatory skewing. The obtained data demonstrated the effect of an anti-inflammatory shift in the T-helper response after transplantation of sterile stool filtrate, which increased and persisted for at least three months after the procedure.

KW - immune response

KW - regulatory T cells

KW - sterile fecal microbiota transplantation

KW - ulcerative colitis

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85184739918&origin=inward&txGid=2261c2c805cd52829d0dd5ad029c9f25

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:001160121600001

UR - https://www.mendeley.com/catalogue/24544b9a-5e2c-3eb8-a03e-020e96e6928b/

U2 - 10.3390/ijms25031886

DO - 10.3390/ijms25031886

M3 - Article

C2 - 38339169

VL - 25

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 3

M1 - 1886

ER -