Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins. / Naumova, Olga; Generalov, Vladimir; Shcherbakov, Dmitry и др.
в: Biosensors, Том 12, № 11, 08.11.2022.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins
AU - Naumova, Olga
AU - Generalov, Vladimir
AU - Shcherbakov, Dmitry
AU - Zaitseva, Elza
AU - Zhivodkov, Yuriy
AU - Kozhukhov, Anton
AU - Latyshev, Alexander
AU - Aseev, Alexander
AU - Safatov, Alexander
AU - Buryak, Galina
AU - Cheremiskina, Anastasia
AU - Merkuleva, Julia
AU - Rudometova, Nadezhda
N1 - Funding: This research was funded by the Russian Foundation for Basic Research (grant number 18-29-02091) and the State assignment of Rospotrebnadzor Russia.
PY - 2022/11/8
Y1 - 2022/11/8
N2 - Quick label-free virus screening and highly sensitive analytical tools/techniques are becoming extremely important in a pandemic. In this study, we developed a biosensing device based on the silicon nanoribbon multichannel and dielectrophoretic controlled sensors functionalized with SARS-CoV-2 spike antibodies for the use as a platform for the detection and studding of properties of viruses and their protein components. Replicatively defective viral particles based on vesicular stomatitis viruses and HIV-1 were used as carrier molecules to deliver the target SARS-CoV-2 spike S-proteins to sensory elements. It was shown that fully CMOS-compatible nanoribbon sensors have the subattomolar sensitivity and dynamic range of 4 orders. Specific interaction between S-proteins and antibodies leads to the accumulation of the negative charge on the sensor surface. Nonspecific interactions of the viral particles lead to the positive charge accumulation. It was shown that dielectrophoretic controlled sensors allow to estimate the effective charge of the single virus at the sensor surface and separate it from the charge associated with the binding of target proteins with the sensor surface.
AB - Quick label-free virus screening and highly sensitive analytical tools/techniques are becoming extremely important in a pandemic. In this study, we developed a biosensing device based on the silicon nanoribbon multichannel and dielectrophoretic controlled sensors functionalized with SARS-CoV-2 spike antibodies for the use as a platform for the detection and studding of properties of viruses and their protein components. Replicatively defective viral particles based on vesicular stomatitis viruses and HIV-1 were used as carrier molecules to deliver the target SARS-CoV-2 spike S-proteins to sensory elements. It was shown that fully CMOS-compatible nanoribbon sensors have the subattomolar sensitivity and dynamic range of 4 orders. Specific interaction between S-proteins and antibodies leads to the accumulation of the negative charge on the sensor surface. Nonspecific interactions of the viral particles lead to the positive charge accumulation. It was shown that dielectrophoretic controlled sensors allow to estimate the effective charge of the single virus at the sensor surface and separate it from the charge associated with the binding of target proteins with the sensor surface.
KW - biosensor
KW - dielectrophoresis
KW - SARS-CoV-2
KW - SOI-FET
KW - spike protein
UR - http://www.scopus.com/inward/record.url?scp=85141892755&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/3eedd401-b31f-3d92-803e-0ff9c5cc001c/
U2 - 10.3390/bios12110992
DO - 10.3390/bios12110992
M3 - Article
C2 - 36354501
AN - SCOPUS:85141892755
VL - 12
JO - Biosensors
JF - Biosensors
SN - 2079-6374
IS - 11
ER -
ID: 39467369