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SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins. / Naumova, Olga; Generalov, Vladimir; Shcherbakov, Dmitry и др.

в: Biosensors, Том 12, № 11, 08.11.2022.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Naumova, O, Generalov, V, Shcherbakov, D, Zaitseva, E, Zhivodkov, Y, Kozhukhov, A, Latyshev, A, Aseev, A, Safatov, A, Buryak, G, Cheremiskina, A, Merkuleva, J & Rudometova, N 2022, 'SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins', Biosensors, Том. 12, № 11. https://doi.org/10.3390/bios12110992

APA

Naumova, O., Generalov, V., Shcherbakov, D., Zaitseva, E., Zhivodkov, Y., Kozhukhov, A., Latyshev, A., Aseev, A., Safatov, A., Buryak, G., Cheremiskina, A., Merkuleva, J., & Rudometova, N. (2022). SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins. Biosensors, 12(11). https://doi.org/10.3390/bios12110992

Vancouver

Naumova O, Generalov V, Shcherbakov D, Zaitseva E, Zhivodkov Y, Kozhukhov A и др. SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins. Biosensors. 2022 нояб. 8;12(11). doi: 10.3390/bios12110992

Author

Naumova, Olga ; Generalov, Vladimir ; Shcherbakov, Dmitry и др. / SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins. в: Biosensors. 2022 ; Том 12, № 11.

BibTeX

@article{24fed7d3cd5d4290b8c00b3b66e5cc1b,
title = "SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins",
abstract = "Quick label-free virus screening and highly sensitive analytical tools/techniques are becoming extremely important in a pandemic. In this study, we developed a biosensing device based on the silicon nanoribbon multichannel and dielectrophoretic controlled sensors functionalized with SARS-CoV-2 spike antibodies for the use as a platform for the detection and studding of properties of viruses and their protein components. Replicatively defective viral particles based on vesicular stomatitis viruses and HIV-1 were used as carrier molecules to deliver the target SARS-CoV-2 spike S-proteins to sensory elements. It was shown that fully CMOS-compatible nanoribbon sensors have the subattomolar sensitivity and dynamic range of 4 orders. Specific interaction between S-proteins and antibodies leads to the accumulation of the negative charge on the sensor surface. Nonspecific interactions of the viral particles lead to the positive charge accumulation. It was shown that dielectrophoretic controlled sensors allow to estimate the effective charge of the single virus at the sensor surface and separate it from the charge associated with the binding of target proteins with the sensor surface.",
keywords = "biosensor, dielectrophoresis, SARS-CoV-2, SOI-FET, spike protein",
author = "Olga Naumova and Vladimir Generalov and Dmitry Shcherbakov and Elza Zaitseva and Yuriy Zhivodkov and Anton Kozhukhov and Alexander Latyshev and Alexander Aseev and Alexander Safatov and Galina Buryak and Anastasia Cheremiskina and Julia Merkuleva and Nadezhda Rudometova",
note = "Funding: This research was funded by the Russian Foundation for Basic Research (grant number 18-29-02091) and the State assignment of Rospotrebnadzor Russia.",
year = "2022",
month = nov,
day = "8",
doi = "10.3390/bios12110992",
language = "English",
volume = "12",
journal = "Biosensors",
issn = "2079-6374",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "11",

}

RIS

TY - JOUR

T1 - SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins

AU - Naumova, Olga

AU - Generalov, Vladimir

AU - Shcherbakov, Dmitry

AU - Zaitseva, Elza

AU - Zhivodkov, Yuriy

AU - Kozhukhov, Anton

AU - Latyshev, Alexander

AU - Aseev, Alexander

AU - Safatov, Alexander

AU - Buryak, Galina

AU - Cheremiskina, Anastasia

AU - Merkuleva, Julia

AU - Rudometova, Nadezhda

N1 - Funding: This research was funded by the Russian Foundation for Basic Research (grant number 18-29-02091) and the State assignment of Rospotrebnadzor Russia.

PY - 2022/11/8

Y1 - 2022/11/8

N2 - Quick label-free virus screening and highly sensitive analytical tools/techniques are becoming extremely important in a pandemic. In this study, we developed a biosensing device based on the silicon nanoribbon multichannel and dielectrophoretic controlled sensors functionalized with SARS-CoV-2 spike antibodies for the use as a platform for the detection and studding of properties of viruses and their protein components. Replicatively defective viral particles based on vesicular stomatitis viruses and HIV-1 were used as carrier molecules to deliver the target SARS-CoV-2 spike S-proteins to sensory elements. It was shown that fully CMOS-compatible nanoribbon sensors have the subattomolar sensitivity and dynamic range of 4 orders. Specific interaction between S-proteins and antibodies leads to the accumulation of the negative charge on the sensor surface. Nonspecific interactions of the viral particles lead to the positive charge accumulation. It was shown that dielectrophoretic controlled sensors allow to estimate the effective charge of the single virus at the sensor surface and separate it from the charge associated with the binding of target proteins with the sensor surface.

AB - Quick label-free virus screening and highly sensitive analytical tools/techniques are becoming extremely important in a pandemic. In this study, we developed a biosensing device based on the silicon nanoribbon multichannel and dielectrophoretic controlled sensors functionalized with SARS-CoV-2 spike antibodies for the use as a platform for the detection and studding of properties of viruses and their protein components. Replicatively defective viral particles based on vesicular stomatitis viruses and HIV-1 were used as carrier molecules to deliver the target SARS-CoV-2 spike S-proteins to sensory elements. It was shown that fully CMOS-compatible nanoribbon sensors have the subattomolar sensitivity and dynamic range of 4 orders. Specific interaction between S-proteins and antibodies leads to the accumulation of the negative charge on the sensor surface. Nonspecific interactions of the viral particles lead to the positive charge accumulation. It was shown that dielectrophoretic controlled sensors allow to estimate the effective charge of the single virus at the sensor surface and separate it from the charge associated with the binding of target proteins with the sensor surface.

KW - biosensor

KW - dielectrophoresis

KW - SARS-CoV-2

KW - SOI-FET

KW - spike protein

UR - http://www.scopus.com/inward/record.url?scp=85141892755&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/3eedd401-b31f-3d92-803e-0ff9c5cc001c/

U2 - 10.3390/bios12110992

DO - 10.3390/bios12110992

M3 - Article

C2 - 36354501

AN - SCOPUS:85141892755

VL - 12

JO - Biosensors

JF - Biosensors

SN - 2079-6374

IS - 11

ER -

ID: 39467369