Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Single-Cell DNA Methylation Analysis of Chicken Lampbrush Chromosomes. / Nurislamov, Artem; Lagunov, Timofey; Gridina, Maria и др.
в: International Journal of Molecular Sciences, Том 23, № 20, 12601, 20.10.2022.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Single-Cell DNA Methylation Analysis of Chicken Lampbrush Chromosomes
AU - Nurislamov, Artem
AU - Lagunov, Timofey
AU - Gridina, Maria
AU - Krasikova, Alla
AU - Fishman, Veniamin
N1 - Publisher Copyright: © 2022 by the authors.
PY - 2022/10/20
Y1 - 2022/10/20
N2 - DNA methylation is an essential epigenetic regulation mechanism implicated in transcription and replication control, developmental reprogramming, retroelements silencing and other genomic processes. During mammalian development, a specific DNA methylation pattern should be established in germ cells to allow embryonic development. Less is known about germ cell DNA methylation in other species. To close this gap, we performed a single-cell methylome analysis of chicken diplotene oocytes. We comprehensively characterized methylation patterns in these cells, obtained methylation-based chicken genome segmentation and identified oocyte-specific methylated gene promoters. Our data show that despite the formation of specific transcriptionally hyperactive genome architecture in chicken diplotene oocytes, methylation patterns in these cells closely resemble genomic distribution observed in somatic tissues.
AB - DNA methylation is an essential epigenetic regulation mechanism implicated in transcription and replication control, developmental reprogramming, retroelements silencing and other genomic processes. During mammalian development, a specific DNA methylation pattern should be established in germ cells to allow embryonic development. Less is known about germ cell DNA methylation in other species. To close this gap, we performed a single-cell methylome analysis of chicken diplotene oocytes. We comprehensively characterized methylation patterns in these cells, obtained methylation-based chicken genome segmentation and identified oocyte-specific methylated gene promoters. Our data show that despite the formation of specific transcriptionally hyperactive genome architecture in chicken diplotene oocytes, methylation patterns in these cells closely resemble genomic distribution observed in somatic tissues.
KW - chicken genome
KW - chicken oocyte
KW - DNA methylation
KW - lampbrush chromosomes
UR - http://www.scopus.com/inward/record.url?scp=85140814115&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/db8f0f75-9086-3b33-aa28-fd29377e8cc5/
U2 - 10.3390/ijms232012601
DO - 10.3390/ijms232012601
M3 - Article
C2 - 36293454
AN - SCOPUS:85140814115
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 20
M1 - 12601
ER -
ID: 38662469