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Single-Cell DNA Methylation Analysis of Chicken Lampbrush Chromosomes. / Nurislamov, Artem; Lagunov, Timofey; Gridina, Maria et al.

In: International Journal of Molecular Sciences, Vol. 23, No. 20, 12601, 20.10.2022.

Research output: Contribution to journal › Article › peer-review

Harvard

Nurislamov, A, Lagunov, T, Gridina, M, Krasikova, A & Fishman, V 2022, 'Single-Cell DNA Methylation Analysis of Chicken Lampbrush Chromosomes', International Journal of Molecular Sciences, vol. 23, no. 20, 12601. https://doi.org/10.3390/ijms232012601

APA

Nurislamov, A., Lagunov, T., Gridina, M., Krasikova, A., & Fishman, V. (2022). Single-Cell DNA Methylation Analysis of Chicken Lampbrush Chromosomes. International Journal of Molecular Sciences, 23(20), [12601]. https://doi.org/10.3390/ijms232012601

Vancouver

Nurislamov A, Lagunov T, Gridina M, Krasikova A, Fishman V. Single-Cell DNA Methylation Analysis of Chicken Lampbrush Chromosomes. International Journal of Molecular Sciences. 2022 Oct 20;23(20):12601. doi: 10.3390/ijms232012601

Author

Nurislamov, Artem ; Lagunov, Timofey ; Gridina, Maria et al. / Single-Cell DNA Methylation Analysis of Chicken Lampbrush Chromosomes. In: International Journal of Molecular Sciences. 2022 ; Vol. 23, No. 20.

BibTeX

@article{a81243b8dfb74dde8c64ece1673d411e,
title = "Single-Cell DNA Methylation Analysis of Chicken Lampbrush Chromosomes",
abstract = "DNA methylation is an essential epigenetic regulation mechanism implicated in transcription and replication control, developmental reprogramming, retroelements silencing and other genomic processes. During mammalian development, a specific DNA methylation pattern should be established in germ cells to allow embryonic development. Less is known about germ cell DNA methylation in other species. To close this gap, we performed a single-cell methylome analysis of chicken diplotene oocytes. We comprehensively characterized methylation patterns in these cells, obtained methylation-based chicken genome segmentation and identified oocyte-specific methylated gene promoters. Our data show that despite the formation of specific transcriptionally hyperactive genome architecture in chicken diplotene oocytes, methylation patterns in these cells closely resemble genomic distribution observed in somatic tissues.",
keywords = "chicken genome, chicken oocyte, DNA methylation, lampbrush chromosomes",
author = "Artem Nurislamov and Timofey Lagunov and Maria Gridina and Alla Krasikova and Veniamin Fishman",
note = "Publisher Copyright: {\textcopyright} 2022 by the authors.",
year = "2022",
month = oct,
day = "20",
doi = "10.3390/ijms232012601",
language = "English",
volume = "23",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "20",

}

RIS

TY - JOUR

T1 - Single-Cell DNA Methylation Analysis of Chicken Lampbrush Chromosomes

AU - Nurislamov, Artem

AU - Lagunov, Timofey

AU - Gridina, Maria

AU - Krasikova, Alla

AU - Fishman, Veniamin

N1 - Publisher Copyright: © 2022 by the authors.

PY - 2022/10/20

Y1 - 2022/10/20

N2 - DNA methylation is an essential epigenetic regulation mechanism implicated in transcription and replication control, developmental reprogramming, retroelements silencing and other genomic processes. During mammalian development, a specific DNA methylation pattern should be established in germ cells to allow embryonic development. Less is known about germ cell DNA methylation in other species. To close this gap, we performed a single-cell methylome analysis of chicken diplotene oocytes. We comprehensively characterized methylation patterns in these cells, obtained methylation-based chicken genome segmentation and identified oocyte-specific methylated gene promoters. Our data show that despite the formation of specific transcriptionally hyperactive genome architecture in chicken diplotene oocytes, methylation patterns in these cells closely resemble genomic distribution observed in somatic tissues.

AB - DNA methylation is an essential epigenetic regulation mechanism implicated in transcription and replication control, developmental reprogramming, retroelements silencing and other genomic processes. During mammalian development, a specific DNA methylation pattern should be established in germ cells to allow embryonic development. Less is known about germ cell DNA methylation in other species. To close this gap, we performed a single-cell methylome analysis of chicken diplotene oocytes. We comprehensively characterized methylation patterns in these cells, obtained methylation-based chicken genome segmentation and identified oocyte-specific methylated gene promoters. Our data show that despite the formation of specific transcriptionally hyperactive genome architecture in chicken diplotene oocytes, methylation patterns in these cells closely resemble genomic distribution observed in somatic tissues.

KW - chicken genome

KW - chicken oocyte

KW - DNA methylation

KW - lampbrush chromosomes

UR - http://www.scopus.com/inward/record.url?scp=85140814115&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/db8f0f75-9086-3b33-aa28-fd29377e8cc5/

U2 - 10.3390/ijms232012601

DO - 10.3390/ijms232012601

M3 - Article

C2 - 36293454

AN - SCOPUS:85140814115

VL - 23

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 20

M1 - 12601

ER -

ID: 38662469