Результаты исследований: Публикации в книгах, отчётах, сборниках, трудах конференций › глава/раздел › научная › Рецензирование
Robust and Reproducible Protocol for Phage Genome "Rebooting" Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid. / Baykov, Ivan; Kurchenko, Olga; Mikhaylova, Ekaterina и др.
Methods in Molecular Biology. ред. / Joana Azeredo; Sanna Sillankorva. Том 2734 2. ред. Humana Press, 2024. стр. 301-317 (Methods in molecular biology (Clifton, N.J.)).Результаты исследований: Публикации в книгах, отчётах, сборниках, трудах конференций › глава/раздел › научная › Рецензирование
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TY - CHAP
T1 - Robust and Reproducible Protocol for Phage Genome "Rebooting" Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid
AU - Baykov, Ivan
AU - Kurchenko, Olga
AU - Mikhaylova, Ekaterina
AU - Morozova, Vera V
AU - Tikunova, Nina V
N1 - This work was supported by the Russian state-funded project for ICBFM SB RAS (grant number 121031300043-8). © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2024
Y1 - 2024
N2 - Production of infectious bacteriophage based on its genome is one of the necessary steps in the pipeline of editing phage genomes and creating synthetic bacteriophages. This process is called "rebooting" of the phage genome. In this chapter, we describe key steps required for successful genome "rebooting" using a native host or intermediate host. A detailed protocol is given for the "rebooting" of the genome of T7 bacteriophage specific to Escherichia coli and bacteriophage KP32_192 that infects Klebsiella pneumoniae.
AB - Production of infectious bacteriophage based on its genome is one of the necessary steps in the pipeline of editing phage genomes and creating synthetic bacteriophages. This process is called "rebooting" of the phage genome. In this chapter, we describe key steps required for successful genome "rebooting" using a native host or intermediate host. A detailed protocol is given for the "rebooting" of the genome of T7 bacteriophage specific to Escherichia coli and bacteriophage KP32_192 that infects Klebsiella pneumoniae.
KW - Gap repair cloning
KW - Genome rebooting
KW - Podovirus
KW - Synthetic bacteriophage
KW - Transformation-associated recombination cloning
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85179647227&origin=inward&txGid=be1e9680aada32d38fc5cfac1e15c593
UR - https://www.mendeley.com/catalogue/4db77924-0872-3106-ad64-68855c4fd51c/
U2 - 10.1007/978-1-0716-3523-0_19
DO - 10.1007/978-1-0716-3523-0_19
M3 - Chapter
C2 - 38066377
SN - 978-1-0716-3522-3
VL - 2734
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 301
EP - 317
BT - Methods in Molecular Biology
A2 - Azeredo, Joana
A2 - Sillankorva, Sanna
PB - Humana Press
ER -
ID: 59334745