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Robust and Reproducible Protocol for Phage Genome "Rebooting" Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid. / Baykov, Ivan; Kurchenko, Olga; Mikhaylova, Ekaterina et al.

Methods in Molecular Biology. ed. / Joana Azeredo; Sanna Sillankorva. Vol. 2734 2. ed. Humana Press, 2024. p. 301-317 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review

Harvard

Baykov, I, Kurchenko, O, Mikhaylova, E, Morozova, VV & Tikunova, NV 2024, Robust and Reproducible Protocol for Phage Genome "Rebooting" Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid. in J Azeredo & S Sillankorva (eds), Methods in Molecular Biology. 2 edn, vol. 2734, Methods in molecular biology (Clifton, N.J.), Humana Press, pp. 301-317. https://doi.org/10.1007/978-1-0716-3523-0_19

APA

Baykov, I., Kurchenko, O., Mikhaylova, E., Morozova, V. V., & Tikunova, N. V. (2024). Robust and Reproducible Protocol for Phage Genome "Rebooting" Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid. In J. Azeredo, & S. Sillankorva (Eds.), Methods in Molecular Biology (2 ed., Vol. 2734, pp. 301-317). (Methods in molecular biology (Clifton, N.J.)). Humana Press. https://doi.org/10.1007/978-1-0716-3523-0_19

Vancouver

Baykov I, Kurchenko O, Mikhaylova E, Morozova VV, Tikunova NV. Robust and Reproducible Protocol for Phage Genome "Rebooting" Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid. In Azeredo J, Sillankorva S, editors, Methods in Molecular Biology. 2 ed. Vol. 2734. Humana Press. 2024. p. 301-317. (Methods in molecular biology (Clifton, N.J.)). Epub 2023 Dec 9. doi: 10.1007/978-1-0716-3523-0_19

Author

Baykov, Ivan ; Kurchenko, Olga ; Mikhaylova, Ekaterina et al. / Robust and Reproducible Protocol for Phage Genome "Rebooting" Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid. Methods in Molecular Biology. editor / Joana Azeredo ; Sanna Sillankorva. Vol. 2734 2. ed. Humana Press, 2024. pp. 301-317 (Methods in molecular biology (Clifton, N.J.)).

BibTeX

@inbook{68eeab21ca03477db44059bdcef32d91,
title = "Robust and Reproducible Protocol for Phage Genome {"}Rebooting{"} Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid",
abstract = "Production of infectious bacteriophage based on its genome is one of the necessary steps in the pipeline of editing phage genomes and creating synthetic bacteriophages. This process is called {"}rebooting{"} of the phage genome. In this chapter, we describe key steps required for successful genome {"}rebooting{"} using a native host or intermediate host. A detailed protocol is given for the {"}rebooting{"} of the genome of T7 bacteriophage specific to Escherichia coli and bacteriophage KP32_192 that infects Klebsiella pneumoniae.",
keywords = "Gap repair cloning, Genome rebooting, Podovirus, Synthetic bacteriophage, Transformation-associated recombination cloning",
author = "Ivan Baykov and Olga Kurchenko and Ekaterina Mikhaylova and Morozova, {Vera V} and Tikunova, {Nina V}",
note = "This work was supported by the Russian state-funded project for ICBFM SB RAS (grant number 121031300043-8). {\textcopyright} 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2024",
doi = "10.1007/978-1-0716-3523-0_19",
language = "English",
isbn = "978-1-0716-3522-3",
volume = "2734",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "301--317",
editor = "Joana Azeredo and Sanna Sillankorva",
booktitle = "Methods in Molecular Biology",
address = "United States",
edition = "2",

}

RIS

TY - CHAP

T1 - Robust and Reproducible Protocol for Phage Genome "Rebooting" Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid

AU - Baykov, Ivan

AU - Kurchenko, Olga

AU - Mikhaylova, Ekaterina

AU - Morozova, Vera V

AU - Tikunova, Nina V

N1 - This work was supported by the Russian state-funded project for ICBFM SB RAS (grant number 121031300043-8). © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2024

Y1 - 2024

N2 - Production of infectious bacteriophage based on its genome is one of the necessary steps in the pipeline of editing phage genomes and creating synthetic bacteriophages. This process is called "rebooting" of the phage genome. In this chapter, we describe key steps required for successful genome "rebooting" using a native host or intermediate host. A detailed protocol is given for the "rebooting" of the genome of T7 bacteriophage specific to Escherichia coli and bacteriophage KP32_192 that infects Klebsiella pneumoniae.

AB - Production of infectious bacteriophage based on its genome is one of the necessary steps in the pipeline of editing phage genomes and creating synthetic bacteriophages. This process is called "rebooting" of the phage genome. In this chapter, we describe key steps required for successful genome "rebooting" using a native host or intermediate host. A detailed protocol is given for the "rebooting" of the genome of T7 bacteriophage specific to Escherichia coli and bacteriophage KP32_192 that infects Klebsiella pneumoniae.

KW - Gap repair cloning

KW - Genome rebooting

KW - Podovirus

KW - Synthetic bacteriophage

KW - Transformation-associated recombination cloning

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85179647227&origin=inward&txGid=be1e9680aada32d38fc5cfac1e15c593

UR - https://www.mendeley.com/catalogue/4db77924-0872-3106-ad64-68855c4fd51c/

U2 - 10.1007/978-1-0716-3523-0_19

DO - 10.1007/978-1-0716-3523-0_19

M3 - Chapter

C2 - 38066377

SN - 978-1-0716-3522-3

VL - 2734

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 301

EP - 317

BT - Methods in Molecular Biology

A2 - Azeredo, Joana

A2 - Sillankorva, Sanna

PB - Humana Press

ER -

ID: 59334745