TY - JOUR

T1 - Regulatory functions and chromatin loading dynamics of linker histone H1 during endoreplication in Drosophila

AU - Andreyeva, Evgeniya N.

AU - Bernardo, Travis J.

AU - Kolesnikova, Tatyana D.

AU - Lu, Xingwu

AU - Yarinich, Lyubov A.

AU - Bartholdy, Boris A.

AU - Guo, Xiaohan

AU - Posukh, Olga V.

AU - Healton, Sean

AU - Willcockson, Michael A.

AU - Pindyurin, Alexey V.

AU - Zhimulev, Igor F.

AU - Skoultchi, Arthur I.

AU - Fyodorov, Dmitry V.

N1 - Publisher Copyright: © 2017 Andreyeva et al.

PY - 2017/3/15

Y1 - 2017/3/15

N2 - Eukaryotic DNA replicates asynchronously, with discrete genomic loci replicating during different stages of S phase. Drosophila larval tissues undergo endoreplication without cell division, and the latest replicating regions occasionally fail to complete endoreplication, resulting in underreplicated domains of polytene chromosomes. Here we show that linker histone H1 is required for the underreplication (UR) phenomenon in Drosophila salivary glands. H1 directly interacts with the Suppressor of UR (SUUR) protein and is required for SUUR binding to chromatin in vivo. These observations implicate H1 as a critical factor in the formation of underreplicated regions and an upstream effector of SUUR. We also demonstrate that the localization of H1 in chromatin changes profoundly during the endocycle. At the onset of endocycle S (endo-S) phase, H1 is heavily and specifically loaded into late replicating genomic regions and is then redistributed during the course of endoreplication. Our data suggest that cell cycle-dependent chromosome occupancy of H1 is governed by several independent processes. In addition to the ubiquitous replication-related disassembly and reassembly of chromatin, H1 is deposited into chromatin through a novel pathway that is replication-independent, rapid, and locus-specific. This cell cycle-directed dynamic localization of H1 in chromatin may play an important role in the regulation of DNA replication timing.

AB - Eukaryotic DNA replicates asynchronously, with discrete genomic loci replicating during different stages of S phase. Drosophila larval tissues undergo endoreplication without cell division, and the latest replicating regions occasionally fail to complete endoreplication, resulting in underreplicated domains of polytene chromosomes. Here we show that linker histone H1 is required for the underreplication (UR) phenomenon in Drosophila salivary glands. H1 directly interacts with the Suppressor of UR (SUUR) protein and is required for SUUR binding to chromatin in vivo. These observations implicate H1 as a critical factor in the formation of underreplicated regions and an upstream effector of SUUR. We also demonstrate that the localization of H1 in chromatin changes profoundly during the endocycle. At the onset of endocycle S (endo-S) phase, H1 is heavily and specifically loaded into late replicating genomic regions and is then redistributed during the course of endoreplication. Our data suggest that cell cycle-dependent chromosome occupancy of H1 is governed by several independent processes. In addition to the ubiquitous replication-related disassembly and reassembly of chromatin, H1 is deposited into chromatin through a novel pathway that is replication-independent, rapid, and locus-specific. This cell cycle-directed dynamic localization of H1 in chromatin may play an important role in the regulation of DNA replication timing.

KW - endoreplication

KW - intercalary heterochromatin

KW - linker histone H1

KW - polytene chromosomes

KW - replication timing

KW - suppressor of underreplication

KW - Heterochromatin/metabolism

KW - Salivary Glands/metabolism

KW - Endoreduplication

KW - Chromatin/metabolism

KW - Drosophila Proteins/metabolism

KW - DNA-Binding Proteins/metabolism

KW - Histones/metabolism

KW - Larva/genetics

KW - Drosophila/genetics

KW - Animals

KW - S Phase/genetics

UR - http://www.scopus.com/inward/record.url?scp=85018609301&partnerID=8YFLogxK

U2 - 10.1101/gad.295717.116

DO - 10.1101/gad.295717.116

M3 - Article

C2 - 28404631

AN - SCOPUS:85018609301

VL - 31

SP - 603

EP - 616

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 6

ER -