Research output: Contribution to journal › Article › peer-review
Regulatory functions and chromatin loading dynamics of linker histone H1 during endoreplication in Drosophila. / Andreyeva, Evgeniya N.; Bernardo, Travis J.; Kolesnikova, Tatyana D. et al.
In: Genes & development, Vol. 31, No. 6, 15.03.2017, p. 603-616.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Regulatory functions and chromatin loading dynamics of linker histone H1 during endoreplication in Drosophila
AU - Andreyeva, Evgeniya N.
AU - Bernardo, Travis J.
AU - Kolesnikova, Tatyana D.
AU - Lu, Xingwu
AU - Yarinich, Lyubov A.
AU - Bartholdy, Boris A.
AU - Guo, Xiaohan
AU - Posukh, Olga V.
AU - Healton, Sean
AU - Willcockson, Michael A.
AU - Pindyurin, Alexey V.
AU - Zhimulev, Igor F.
AU - Skoultchi, Arthur I.
AU - Fyodorov, Dmitry V.
N1 - Publisher Copyright: © 2017 Andreyeva et al.
PY - 2017/3/15
Y1 - 2017/3/15
N2 - Eukaryotic DNA replicates asynchronously, with discrete genomic loci replicating during different stages of S phase. Drosophila larval tissues undergo endoreplication without cell division, and the latest replicating regions occasionally fail to complete endoreplication, resulting in underreplicated domains of polytene chromosomes. Here we show that linker histone H1 is required for the underreplication (UR) phenomenon in Drosophila salivary glands. H1 directly interacts with the Suppressor of UR (SUUR) protein and is required for SUUR binding to chromatin in vivo. These observations implicate H1 as a critical factor in the formation of underreplicated regions and an upstream effector of SUUR. We also demonstrate that the localization of H1 in chromatin changes profoundly during the endocycle. At the onset of endocycle S (endo-S) phase, H1 is heavily and specifically loaded into late replicating genomic regions and is then redistributed during the course of endoreplication. Our data suggest that cell cycle-dependent chromosome occupancy of H1 is governed by several independent processes. In addition to the ubiquitous replication-related disassembly and reassembly of chromatin, H1 is deposited into chromatin through a novel pathway that is replication-independent, rapid, and locus-specific. This cell cycle-directed dynamic localization of H1 in chromatin may play an important role in the regulation of DNA replication timing.
AB - Eukaryotic DNA replicates asynchronously, with discrete genomic loci replicating during different stages of S phase. Drosophila larval tissues undergo endoreplication without cell division, and the latest replicating regions occasionally fail to complete endoreplication, resulting in underreplicated domains of polytene chromosomes. Here we show that linker histone H1 is required for the underreplication (UR) phenomenon in Drosophila salivary glands. H1 directly interacts with the Suppressor of UR (SUUR) protein and is required for SUUR binding to chromatin in vivo. These observations implicate H1 as a critical factor in the formation of underreplicated regions and an upstream effector of SUUR. We also demonstrate that the localization of H1 in chromatin changes profoundly during the endocycle. At the onset of endocycle S (endo-S) phase, H1 is heavily and specifically loaded into late replicating genomic regions and is then redistributed during the course of endoreplication. Our data suggest that cell cycle-dependent chromosome occupancy of H1 is governed by several independent processes. In addition to the ubiquitous replication-related disassembly and reassembly of chromatin, H1 is deposited into chromatin through a novel pathway that is replication-independent, rapid, and locus-specific. This cell cycle-directed dynamic localization of H1 in chromatin may play an important role in the regulation of DNA replication timing.
KW - endoreplication
KW - intercalary heterochromatin
KW - linker histone H1
KW - polytene chromosomes
KW - replication timing
KW - suppressor of underreplication
KW - Heterochromatin/metabolism
KW - Salivary Glands/metabolism
KW - Endoreduplication
KW - Chromatin/metabolism
KW - Drosophila Proteins/metabolism
KW - DNA-Binding Proteins/metabolism
KW - Histones/metabolism
KW - Larva/genetics
KW - Drosophila/genetics
KW - Animals
KW - S Phase/genetics
UR - http://www.scopus.com/inward/record.url?scp=85018609301&partnerID=8YFLogxK
U2 - 10.1101/gad.295717.116
DO - 10.1101/gad.295717.116
M3 - Article
C2 - 28404631
AN - SCOPUS:85018609301
VL - 31
SP - 603
EP - 616
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 6
ER -
ID: 10257416