Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing. / Okotrub, K. A.; Amstislavsky, S. Y.; Surovtsev, N. V.
в: Archives of Biochemistry and Biophysics, Том 635, 01.12.2017, стр. 37-43.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing
AU - Okotrub, K. A.
AU - Amstislavsky, S. Y.
AU - Surovtsev, N. V.
N1 - Publisher Copyright: © 2017 Elsevier Inc.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Although lipid phase transition is believed to be among the major damaging factors in oocytes and preimplantation embryos cryopreservation, lack of the appropriate experimental methods limits investigation of this phenomenon. Herein, we demonstrate the capabilities of Raman spectroscopy to detect the lipid phase transition within the freezing preimplantation mouse embryos. We exploit the sensibility of antisymmetric CH2 Raman peak to the phase state of lipids. It is shown that during the freezing of the mouse embryos the lipid phase transition occurs at the temperatures between −7 and 0 °C. Similar temperature dependences of CH2 mode intensities are found for lipids in the preimplantation embryos and a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, implying the similarity in the occupation rules of conformational states. Raman spectroscopy is considered as a method of choice to study the lipid phase transition during preimplantation mammalian embryos freezing and cryopreservation.
AB - Although lipid phase transition is believed to be among the major damaging factors in oocytes and preimplantation embryos cryopreservation, lack of the appropriate experimental methods limits investigation of this phenomenon. Herein, we demonstrate the capabilities of Raman spectroscopy to detect the lipid phase transition within the freezing preimplantation mouse embryos. We exploit the sensibility of antisymmetric CH2 Raman peak to the phase state of lipids. It is shown that during the freezing of the mouse embryos the lipid phase transition occurs at the temperatures between −7 and 0 °C. Similar temperature dependences of CH2 mode intensities are found for lipids in the preimplantation embryos and a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, implying the similarity in the occupation rules of conformational states. Raman spectroscopy is considered as a method of choice to study the lipid phase transition during preimplantation mammalian embryos freezing and cryopreservation.
KW - Cryopreservation
KW - Glycerol
KW - Lipid phase transition
KW - Preimplantation mouse embryo
KW - Program freezing
KW - Raman spectroscopy
KW - Molecular Conformation
KW - Lipids/chemistry
KW - Phase Transition
KW - Blastocyst/chemistry
KW - Cryopreservation/methods
KW - Freezing
KW - Animals
KW - Lipid Bilayers/chemistry
KW - Phosphatidylcholines/chemistry
KW - Female
KW - Mice
KW - Spectrum Analysis, Raman/methods
KW - POLYMETHYLENE CHAIN
KW - H STRETCHING REGION
KW - CRYOPRESERVATION
KW - FROZEN YEAST-CELLS
KW - OOCYTES
KW - CHILLING SENSITIVITY
KW - DEVELOPMENTAL COMPETENCE
KW - INTRACELLULAR ICE FORMATION
KW - FATTY-ACID
KW - IN-VIVO
UR - http://www.scopus.com/inward/record.url?scp=85032034640&partnerID=8YFLogxK
U2 - 10.1016/j.abb.2017.10.001
DO - 10.1016/j.abb.2017.10.001
M3 - Article
C2 - 28993092
AN - SCOPUS:85032034640
VL - 635
SP - 37
EP - 43
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
ER -
ID: 9409716