Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing

Standard

Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing. / Okotrub, K. A.; Amstislavsky, S. Y.; Surovtsev, N. V.

In: Archives of Biochemistry and Biophysics, Vol. 635, 01.12.2017, p. 37-43.

Research output: Contribution to journal › Article › peer-review

Harvard

Okotrub, KA, Amstislavsky, SY & Surovtsev, NV 2017, 'Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing', Archives of Biochemistry and Biophysics, vol. 635, pp. 37-43. https://doi.org/10.1016/j.abb.2017.10.001

APA

Okotrub, K. A., Amstislavsky, S. Y., & Surovtsev, N. V. (2017). Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing. Archives of Biochemistry and Biophysics, 635, 37-43. https://doi.org/10.1016/j.abb.2017.10.001

Vancouver

Okotrub KA, Amstislavsky SY, Surovtsev NV. Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing. Archives of Biochemistry and Biophysics. 2017 Dec 1;635:37-43. doi: 10.1016/j.abb.2017.10.001

Author

Okotrub, K. A. ; Amstislavsky, S. Y. ; Surovtsev, N. V. / Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing. In: Archives of Biochemistry and Biophysics. 2017 ; Vol. 635. pp. 37-43.

BibTeX

@article{0ba731f345af41f0ac250da13ea60918,

title = "Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing",

abstract = "Although lipid phase transition is believed to be among the major damaging factors in oocytes and preimplantation embryos cryopreservation, lack of the appropriate experimental methods limits investigation of this phenomenon. Herein, we demonstrate the capabilities of Raman spectroscopy to detect the lipid phase transition within the freezing preimplantation mouse embryos. We exploit the sensibility of antisymmetric CH2 Raman peak to the phase state of lipids. It is shown that during the freezing of the mouse embryos the lipid phase transition occurs at the temperatures between −7 and 0 °C. Similar temperature dependences of CH2 mode intensities are found for lipids in the preimplantation embryos and a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, implying the similarity in the occupation rules of conformational states. Raman spectroscopy is considered as a method of choice to study the lipid phase transition during preimplantation mammalian embryos freezing and cryopreservation.",

keywords = "Cryopreservation, Glycerol, Lipid phase transition, Preimplantation mouse embryo, Program freezing, Raman spectroscopy, Molecular Conformation, Lipids/chemistry, Phase Transition, Blastocyst/chemistry, Cryopreservation/methods, Freezing, Animals, Lipid Bilayers/chemistry, Phosphatidylcholines/chemistry, Female, Mice, Spectrum Analysis, Raman/methods, POLYMETHYLENE CHAIN, H STRETCHING REGION, CRYOPRESERVATION, FROZEN YEAST-CELLS, OOCYTES, CHILLING SENSITIVITY, DEVELOPMENTAL COMPETENCE, INTRACELLULAR ICE FORMATION, FATTY-ACID, IN-VIVO",

author = "Okotrub, {K. A.} and Amstislavsky, {S. Y.} and Surovtsev, {N. V.}",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

year = "2017",

month = dec,

day = "1",

doi = "10.1016/j.abb.2017.10.001",

language = "English",

volume = "635",

pages = "37--43",

journal = "Archives of Biochemistry and Biophysics",

issn = "0003-9861",

publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Raman spectroscopy reveals the lipid phase transition in preimplantation mouse embryos during freezing

AU - Okotrub, K. A.

AU - Amstislavsky, S. Y.

AU - Surovtsev, N. V.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Although lipid phase transition is believed to be among the major damaging factors in oocytes and preimplantation embryos cryopreservation, lack of the appropriate experimental methods limits investigation of this phenomenon. Herein, we demonstrate the capabilities of Raman spectroscopy to detect the lipid phase transition within the freezing preimplantation mouse embryos. We exploit the sensibility of antisymmetric CH2 Raman peak to the phase state of lipids. It is shown that during the freezing of the mouse embryos the lipid phase transition occurs at the temperatures between −7 and 0 °C. Similar temperature dependences of CH2 mode intensities are found for lipids in the preimplantation embryos and a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, implying the similarity in the occupation rules of conformational states. Raman spectroscopy is considered as a method of choice to study the lipid phase transition during preimplantation mammalian embryos freezing and cryopreservation.

AB - Although lipid phase transition is believed to be among the major damaging factors in oocytes and preimplantation embryos cryopreservation, lack of the appropriate experimental methods limits investigation of this phenomenon. Herein, we demonstrate the capabilities of Raman spectroscopy to detect the lipid phase transition within the freezing preimplantation mouse embryos. We exploit the sensibility of antisymmetric CH2 Raman peak to the phase state of lipids. It is shown that during the freezing of the mouse embryos the lipid phase transition occurs at the temperatures between −7 and 0 °C. Similar temperature dependences of CH2 mode intensities are found for lipids in the preimplantation embryos and a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, implying the similarity in the occupation rules of conformational states. Raman spectroscopy is considered as a method of choice to study the lipid phase transition during preimplantation mammalian embryos freezing and cryopreservation.

KW - Cryopreservation

KW - Glycerol

KW - Lipid phase transition

KW - Preimplantation mouse embryo

KW - Program freezing

KW - Raman spectroscopy

KW - Molecular Conformation

KW - Lipids/chemistry

KW - Phase Transition

KW - Blastocyst/chemistry

KW - Cryopreservation/methods

KW - Freezing

KW - Animals

KW - Lipid Bilayers/chemistry

KW - Phosphatidylcholines/chemistry

KW - Female

KW - Mice

KW - Spectrum Analysis, Raman/methods

KW - POLYMETHYLENE CHAIN

KW - H STRETCHING REGION

KW - CRYOPRESERVATION

KW - FROZEN YEAST-CELLS

KW - OOCYTES

KW - CHILLING SENSITIVITY

KW - DEVELOPMENTAL COMPETENCE

KW - INTRACELLULAR ICE FORMATION

KW - FATTY-ACID

KW - IN-VIVO

UR - http://www.scopus.com/inward/record.url?scp=85032034640&partnerID=8YFLogxK

U2 - 10.1016/j.abb.2017.10.001

DO - 10.1016/j.abb.2017.10.001

M3 - Article

C2 - 28993092

AN - SCOPUS:85032034640

VL - 635

SP - 37

EP - 43

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

ER -

ID: 9409716