Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells. / Ilyin, Artem A.; Ryazansky, Sergei S.; Doronin, Semen A. и др.
в: Nucleic Acids Research, Том 45, № 13, 01.07.2017, стр. 7666-7680.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells
AU - Ilyin, Artem A.
AU - Ryazansky, Sergei S.
AU - Doronin, Semen A.
AU - Olenkina, Oxana M.
AU - Mikhaleva, Elena A.
AU - Yakushev, Evgeny Y.
AU - Abramov, Yuri A.
AU - Belyakin, Stepan N.
AU - Ivankin, Anton V.
AU - Pindyurin, Alexey V.
AU - Gvozdev, Vladimir A.
AU - Klenov, Mikhail S.
AU - Shevelyov, Yuri Y.
N1 - © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwimust scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scanning is currently unknown. Herewe report the DamIDseq mapping of multiple Piwi-interacting chromosomal domains in somatic cells of Drosophila ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes (NPCs). Accordingly, Piwi was coimmunoprecipitated with the component of NPCs Elys and with the Xmas-2 subunit of RNA transcription and export complex, known to interact with NPCs. However, only a small Piwi fraction has transient access to DNA at nuclear pores. Importantly, although 36% of the proteincoding genes overlap with Piwi-interacting domains and RNA-immunoprecipitation results demonstrate promiscuous Piwi binding to numerous genic and TE nuclear transcripts, according to available data Piwi does not silence these genes, likely due to the absence of perfect base-pairing between piRNAs and their transcripts.
AB - Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwimust scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scanning is currently unknown. Herewe report the DamIDseq mapping of multiple Piwi-interacting chromosomal domains in somatic cells of Drosophila ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes (NPCs). Accordingly, Piwi was coimmunoprecipitated with the component of NPCs Elys and with the Xmas-2 subunit of RNA transcription and export complex, known to interact with NPCs. However, only a small Piwi fraction has transient access to DNA at nuclear pores. Importantly, although 36% of the proteincoding genes overlap with Piwi-interacting domains and RNA-immunoprecipitation results demonstrate promiscuous Piwi binding to numerous genic and TE nuclear transcripts, according to available data Piwi does not silence these genes, likely due to the absence of perfect base-pairing between piRNAs and their transcripts.
KW - Animals
KW - Animals, Genetically Modified
KW - Argonaute Proteins/chemistry
KW - Chromatin/genetics
KW - DNA Transposable Elements
KW - Drosophila Proteins/chemistry
KW - Drosophila melanogaster/genetics
KW - Female
KW - Gene Silencing
KW - Genome, Insect
KW - Genomic Instability
KW - Models, Biological
KW - Nuclear Pore/genetics
KW - Ovary/cytology
KW - Protein Binding
KW - Protein Interaction Domains and Motifs
KW - RNA, Messenger/genetics
KW - PROTEIN
KW - GERM-LINE
KW - GENE-EXPRESSION
KW - STEM-CELLS
KW - THO COMPLEX
KW - MESSENGER-RNA EXPORT
KW - GENOME
KW - PRIMARY PIRNA BIOGENESIS
KW - SEQUENCING DATA
KW - MELANOGASTER
UR - http://www.scopus.com/inward/record.url?scp=85026434485&partnerID=8YFLogxK
U2 - 10.1093/nar/gkx355
DO - 10.1093/nar/gkx355
M3 - Article
C2 - 28472469
AN - SCOPUS:85026434485
VL - 45
SP - 7666
EP - 7680
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 13
ER -
ID: 10522211