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Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells. / Ilyin, Artem A.; Ryazansky, Sergei S.; Doronin, Semen A. et al.

In: Nucleic Acids Research, Vol. 45, No. 13, 01.07.2017, p. 7666-7680.

Research output: Contribution to journal › Article › peer-review

Harvard

Ilyin, AA, Ryazansky, SS, Doronin, SA, Olenkina, OM, Mikhaleva, EA, Yakushev, EY, Abramov, YA, Belyakin, SN, Ivankin, AV, Pindyurin, AV, Gvozdev, VA, Klenov, MS & Shevelyov, YY 2017, 'Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells', Nucleic Acids Research, vol. 45, no. 13, pp. 7666-7680. https://doi.org/10.1093/nar/gkx355

APA

Ilyin, A. A., Ryazansky, S. S., Doronin, S. A., Olenkina, O. M., Mikhaleva, E. A., Yakushev, E. Y., Abramov, Y. A., Belyakin, S. N., Ivankin, A. V., Pindyurin, A. V., Gvozdev, V. A., Klenov, M. S., & Shevelyov, Y. Y. (2017). Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells. Nucleic Acids Research, 45(13), 7666-7680. https://doi.org/10.1093/nar/gkx355

Vancouver

Ilyin AA, Ryazansky SS, Doronin SA, Olenkina OM, Mikhaleva EA, Yakushev EY et al. Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells. Nucleic Acids Research. 2017 Jul 1;45(13):7666-7680. doi: 10.1093/nar/gkx355

Author

Ilyin, Artem A. ; Ryazansky, Sergei S. ; Doronin, Semen A. et al. / Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells. In: Nucleic Acids Research. 2017 ; Vol. 45, No. 13. pp. 7666-7680.

BibTeX

@article{723031ec19564b0a9017a280827fd260,
title = "Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells",
abstract = "Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwimust scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scanning is currently unknown. Herewe report the DamIDseq mapping of multiple Piwi-interacting chromosomal domains in somatic cells of Drosophila ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes (NPCs). Accordingly, Piwi was coimmunoprecipitated with the component of NPCs Elys and with the Xmas-2 subunit of RNA transcription and export complex, known to interact with NPCs. However, only a small Piwi fraction has transient access to DNA at nuclear pores. Importantly, although 36% of the proteincoding genes overlap with Piwi-interacting domains and RNA-immunoprecipitation results demonstrate promiscuous Piwi binding to numerous genic and TE nuclear transcripts, according to available data Piwi does not silence these genes, likely due to the absence of perfect base-pairing between piRNAs and their transcripts.",
keywords = "Animals, Animals, Genetically Modified, Argonaute Proteins/chemistry, Chromatin/genetics, DNA Transposable Elements, Drosophila Proteins/chemistry, Drosophila melanogaster/genetics, Female, Gene Silencing, Genome, Insect, Genomic Instability, Models, Biological, Nuclear Pore/genetics, Ovary/cytology, Protein Binding, Protein Interaction Domains and Motifs, RNA, Messenger/genetics, PROTEIN, GERM-LINE, GENE-EXPRESSION, STEM-CELLS, THO COMPLEX, MESSENGER-RNA EXPORT, GENOME, PRIMARY PIRNA BIOGENESIS, SEQUENCING DATA, MELANOGASTER",
author = "Ilyin, {Artem A.} and Ryazansky, {Sergei S.} and Doronin, {Semen A.} and Olenkina, {Oxana M.} and Mikhaleva, {Elena A.} and Yakushev, {Evgeny Y.} and Abramov, {Yuri A.} and Belyakin, {Stepan N.} and Ivankin, {Anton V.} and Pindyurin, {Alexey V.} and Gvozdev, {Vladimir A.} and Klenov, {Mikhail S.} and Shevelyov, {Yuri Y.}",
note = "{\textcopyright} The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2017",
month = jul,
day = "1",
doi = "10.1093/nar/gkx355",
language = "English",
volume = "45",
pages = "7666--7680",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "13",

}

RIS

TY - JOUR

T1 - Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells

AU - Ilyin, Artem A.

AU - Ryazansky, Sergei S.

AU - Doronin, Semen A.

AU - Olenkina, Oxana M.

AU - Mikhaleva, Elena A.

AU - Yakushev, Evgeny Y.

AU - Abramov, Yuri A.

AU - Belyakin, Stepan N.

AU - Ivankin, Anton V.

AU - Pindyurin, Alexey V.

AU - Gvozdev, Vladimir A.

AU - Klenov, Mikhail S.

AU - Shevelyov, Yuri Y.

N1 - © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwimust scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scanning is currently unknown. Herewe report the DamIDseq mapping of multiple Piwi-interacting chromosomal domains in somatic cells of Drosophila ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes (NPCs). Accordingly, Piwi was coimmunoprecipitated with the component of NPCs Elys and with the Xmas-2 subunit of RNA transcription and export complex, known to interact with NPCs. However, only a small Piwi fraction has transient access to DNA at nuclear pores. Importantly, although 36% of the proteincoding genes overlap with Piwi-interacting domains and RNA-immunoprecipitation results demonstrate promiscuous Piwi binding to numerous genic and TE nuclear transcripts, according to available data Piwi does not silence these genes, likely due to the absence of perfect base-pairing between piRNAs and their transcripts.

AB - Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwimust scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scanning is currently unknown. Herewe report the DamIDseq mapping of multiple Piwi-interacting chromosomal domains in somatic cells of Drosophila ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes (NPCs). Accordingly, Piwi was coimmunoprecipitated with the component of NPCs Elys and with the Xmas-2 subunit of RNA transcription and export complex, known to interact with NPCs. However, only a small Piwi fraction has transient access to DNA at nuclear pores. Importantly, although 36% of the proteincoding genes overlap with Piwi-interacting domains and RNA-immunoprecipitation results demonstrate promiscuous Piwi binding to numerous genic and TE nuclear transcripts, according to available data Piwi does not silence these genes, likely due to the absence of perfect base-pairing between piRNAs and their transcripts.

KW - Animals

KW - Animals, Genetically Modified

KW - Argonaute Proteins/chemistry

KW - Chromatin/genetics

KW - DNA Transposable Elements

KW - Drosophila Proteins/chemistry

KW - Drosophila melanogaster/genetics

KW - Female

KW - Gene Silencing

KW - Genome, Insect

KW - Genomic Instability

KW - Models, Biological

KW - Nuclear Pore/genetics

KW - Ovary/cytology

KW - Protein Binding

KW - Protein Interaction Domains and Motifs

KW - RNA, Messenger/genetics

KW - PROTEIN

KW - GERM-LINE

KW - GENE-EXPRESSION

KW - STEM-CELLS

KW - THO COMPLEX

KW - MESSENGER-RNA EXPORT

KW - GENOME

KW - PRIMARY PIRNA BIOGENESIS

KW - SEQUENCING DATA

KW - MELANOGASTER

UR - http://www.scopus.com/inward/record.url?scp=85026434485&partnerID=8YFLogxK

U2 - 10.1093/nar/gkx355

DO - 10.1093/nar/gkx355

M3 - Article

C2 - 28472469

AN - SCOPUS:85026434485

VL - 45

SP - 7666

EP - 7680

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 13

ER -

ID: 10522211