TY - JOUR

T1 - Ovothiol a is the main antioxidant in fish lens

AU - Yanshole, Vadim V.

AU - Yanshole, Lyudmila V.

AU - Zelentsova, Ekaterina A.

AU - Tsentalovich, Yuri P.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Tissue protection from oxidative stress by antioxidants is of vital importance for cellular metabolism. The lens mostly consists of fiber cells lacking nuclei and organelles, having minimal metabolic activity; therefore, the defense of the lens tissue from the oxidative stress strongly relies on metabolites. Protein-free extracts from lenses and gills of freshwater fish, Sander lucioperca and Rutilus rutilus lacustris, were subjected to analysis using high-field 1H NMR spectroscopy and HPLC with optical and high-resolution mass spectrometric detection. It was found that the eye lenses of freshwater fish contain high concentrations of ovothiol A (OSH), i.e., one of the most powerful antioxidants exciting in nature. OSH was identified and quantified in millimolar concentrations. The concentration of OSH in the lens and gills depends on the fish genus and on the season. A possible mechanism of the reactive oxygen species deactivation in fish lenses is discussed. This work is the first to report on the presence of OSH in vertebrates. The presence of ovothiol in the fish tissue implies that it may be a significantly more common antioxidant in freshwater and marine animals than was previously thought.

AB - Tissue protection from oxidative stress by antioxidants is of vital importance for cellular metabolism. The lens mostly consists of fiber cells lacking nuclei and organelles, having minimal metabolic activity; therefore, the defense of the lens tissue from the oxidative stress strongly relies on metabolites. Protein-free extracts from lenses and gills of freshwater fish, Sander lucioperca and Rutilus rutilus lacustris, were subjected to analysis using high-field 1H NMR spectroscopy and HPLC with optical and high-resolution mass spectrometric detection. It was found that the eye lenses of freshwater fish contain high concentrations of ovothiol A (OSH), i.e., one of the most powerful antioxidants exciting in nature. OSH was identified and quantified in millimolar concentrations. The concentration of OSH in the lens and gills depends on the fish genus and on the season. A possible mechanism of the reactive oxygen species deactivation in fish lenses is discussed. This work is the first to report on the presence of OSH in vertebrates. The presence of ovothiol in the fish tissue implies that it may be a significantly more common antioxidant in freshwater and marine animals than was previously thought.

KW - Antioxidant

KW - Freshwater fish

KW - Mass spectrometry

KW - NMR spectroscopy

KW - Ovothiol A

KW - antioxidant

KW - SALMO-SALAR L.

KW - ovothiol A

KW - N-ACETYLHISTIDINE

KW - freshwater fish

KW - DISULFIDE

KW - EGGS

KW - THIOLS

KW - AMINO-ACID

KW - METABOLOMICS

KW - GLUTATHIONE

KW - mass spectrometry

KW - ATLANTIC SALMON

UR - http://www.scopus.com/inward/record.url?scp=85070451703&partnerID=8YFLogxK

U2 - 10.3390/metabo9050095

DO - 10.3390/metabo9050095

M3 - Article

C2 - 31083459

AN - SCOPUS:85070451703

VL - 9

JO - Metabolites

JF - Metabolites

SN - 2218-1989

IS - 5

M1 - 95

ER -