Research output: Contribution to journal › Article › peer-review
Ovothiol a is the main antioxidant in fish lens. / Yanshole, Vadim V.; Yanshole, Lyudmila V.; Zelentsova, Ekaterina A. et al.
In: Metabolites, Vol. 9, No. 5, 95, 01.05.2019.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Ovothiol a is the main antioxidant in fish lens
AU - Yanshole, Vadim V.
AU - Yanshole, Lyudmila V.
AU - Zelentsova, Ekaterina A.
AU - Tsentalovich, Yuri P.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - Tissue protection from oxidative stress by antioxidants is of vital importance for cellular metabolism. The lens mostly consists of fiber cells lacking nuclei and organelles, having minimal metabolic activity; therefore, the defense of the lens tissue from the oxidative stress strongly relies on metabolites. Protein-free extracts from lenses and gills of freshwater fish, Sander lucioperca and Rutilus rutilus lacustris, were subjected to analysis using high-field 1H NMR spectroscopy and HPLC with optical and high-resolution mass spectrometric detection. It was found that the eye lenses of freshwater fish contain high concentrations of ovothiol A (OSH), i.e., one of the most powerful antioxidants exciting in nature. OSH was identified and quantified in millimolar concentrations. The concentration of OSH in the lens and gills depends on the fish genus and on the season. A possible mechanism of the reactive oxygen species deactivation in fish lenses is discussed. This work is the first to report on the presence of OSH in vertebrates. The presence of ovothiol in the fish tissue implies that it may be a significantly more common antioxidant in freshwater and marine animals than was previously thought.
AB - Tissue protection from oxidative stress by antioxidants is of vital importance for cellular metabolism. The lens mostly consists of fiber cells lacking nuclei and organelles, having minimal metabolic activity; therefore, the defense of the lens tissue from the oxidative stress strongly relies on metabolites. Protein-free extracts from lenses and gills of freshwater fish, Sander lucioperca and Rutilus rutilus lacustris, were subjected to analysis using high-field 1H NMR spectroscopy and HPLC with optical and high-resolution mass spectrometric detection. It was found that the eye lenses of freshwater fish contain high concentrations of ovothiol A (OSH), i.e., one of the most powerful antioxidants exciting in nature. OSH was identified and quantified in millimolar concentrations. The concentration of OSH in the lens and gills depends on the fish genus and on the season. A possible mechanism of the reactive oxygen species deactivation in fish lenses is discussed. This work is the first to report on the presence of OSH in vertebrates. The presence of ovothiol in the fish tissue implies that it may be a significantly more common antioxidant in freshwater and marine animals than was previously thought.
KW - Antioxidant
KW - Freshwater fish
KW - Mass spectrometry
KW - NMR spectroscopy
KW - Ovothiol A
KW - antioxidant
KW - SALMO-SALAR L.
KW - ovothiol A
KW - N-ACETYLHISTIDINE
KW - freshwater fish
KW - DISULFIDE
KW - EGGS
KW - THIOLS
KW - AMINO-ACID
KW - METABOLOMICS
KW - GLUTATHIONE
KW - mass spectrometry
KW - ATLANTIC SALMON
UR - http://www.scopus.com/inward/record.url?scp=85070451703&partnerID=8YFLogxK
U2 - 10.3390/metabo9050095
DO - 10.3390/metabo9050095
M3 - Article
C2 - 31083459
AN - SCOPUS:85070451703
VL - 9
JO - Metabolites
JF - Metabolites
SN - 2218-1989
IS - 5
M1 - 95
ER -
ID: 21239327