TY - JOUR

T1 - Otu and Rif1 Double Mutant Enables Analysis of Satellite DNA in Polytene Chromosomes of Ovarian Germ Cells in Drosophila melanogaster

AU - Kolesnikova, T D

AU - Nokhova, A R

AU - Shatskikh, A S

AU - Klenov, M S

AU - Zhimulev, I F

N1 - Part of the work related to the morphological analysis of the chromosomes of nurse cells was funded by the Russian Science Foundation (grant no. 19-14-00051-P). Part of the work related to in situ hybridization was funded by the Russian Science Foundation (grant no. 19-14-00382). © 2023. Pleiades Publishing, Ltd.

PY - 2024

Y1 - 2024

N2 - Polytene chromosomes in Drosophila serve as a classical model for cytogenetic studies. However, heterochromatic regions of chromosomes are typically under-replicated, hindering their analysis. Mutations in the Rif1 gene lead to additional replication of heterochromatic sequences, including satellite DNA, in salivary gland cells. Here, we investigated the impact of the Rif1 mutation on heterochromatin in polytene chromosomes formed in ovarian germ cells due to the otu gene mutation. By the analysis of otu11; Rif11 double mutants, we found that, in the presence of the Rif1 mutation, ovarian cells undergo additional polytenization of pericentromeric regions. This includes the formation of large chromatin blocks composed of satellite DNA. Thus, the effects of the Rif1 mutation are similar in salivary gland and germ cells. The otu11; Rif11 system opens new possibilities for studying factors associated with heterochromatin during oogenesis.

AB - Polytene chromosomes in Drosophila serve as a classical model for cytogenetic studies. However, heterochromatic regions of chromosomes are typically under-replicated, hindering their analysis. Mutations in the Rif1 gene lead to additional replication of heterochromatic sequences, including satellite DNA, in salivary gland cells. Here, we investigated the impact of the Rif1 mutation on heterochromatin in polytene chromosomes formed in ovarian germ cells due to the otu gene mutation. By the analysis of otu11; Rif11 double mutants, we found that, in the presence of the Rif1 mutation, ovarian cells undergo additional polytenization of pericentromeric regions. This includes the formation of large chromatin blocks composed of satellite DNA. Thus, the effects of the Rif1 mutation are similar in salivary gland and germ cells. The otu11; Rif11 system opens new possibilities for studying factors associated with heterochromatin during oogenesis.

KW - Animals

KW - Carrier Proteins/genetics

KW - Chromosomes

KW - DNA Replication

KW - DNA, Satellite/genetics

KW - Drosophila/genetics

KW - Drosophila melanogaster/genetics

KW - Drosophila Proteins/genetics

KW - Germ Cells

KW - Heterochromatin/genetics

KW - Polytene Chromosomes/genetics

KW - Rif1

KW - heterochromatin

KW - satellite DNA

KW - polytene chromosomes

KW - under-replication

KW - pseudonurse cells

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85184919974&origin=inward&txGid=ca04966e4026ccc7640e7566382d60a3

UR - https://www.mendeley.com/catalogue/b00dfdbd-b564-3e17-9b5e-16e39e92e337/

U2 - 10.1134/S160767292360046X

DO - 10.1134/S160767292360046X

M3 - Article

C2 - 38337102

VL - 513

SP - S87-S91

JO - Doklady Biochemistry and Biophysics

JF - Doklady Biochemistry and Biophysics

SN - 1607-6729

IS - Suppl 1

ER -